TY - JOUR
T1 - Theoretical and in vitro studies of a C-terminal peptide from PGKC of Leishmania mexicana mexicana
AU - Kaushik, Sandeep
AU - Krishnarjuna, Bankala
AU - Raghothama, Srinivasarao
AU - Aggarwal, Sangita
AU - Raghunathan, Vidya
AU - Ganjiwale, Anjali
PY - 2012/9/1
Y1 - 2012/9/1
N2 - Trypanosomatids cause deadly diseases in humans. Of the various biochemical pathways in trypanosomatids, glycolysis, has received special attention because of being sequestered in peroxisome like organelles critical for the survival of the parasites. This study focuses on phosphoglycerate kinase (PGK) from Leishmania spp. which, exists in two isoforms, the cytoplasmic PGKB and glycosomal PGKC differing in their biochemical properties. Computational analysis predicted the likelihood of a transmembrane helix only in the glycosomal isoform PGKC, of approximate length 20 residues in the 62-residue extension, ending at, arginine residues R471 and R472. From experimental studies using circular dichroism and NMR with deuterated sodium dodecyl sulfate, we find that the transmembrane helix spans residues 448 ± 2 to 476 in Leishmania mexicana PGKC. The significance of this observation is discussed in the context of glycosomal transport and substrate tunneling.
AB - Trypanosomatids cause deadly diseases in humans. Of the various biochemical pathways in trypanosomatids, glycolysis, has received special attention because of being sequestered in peroxisome like organelles critical for the survival of the parasites. This study focuses on phosphoglycerate kinase (PGK) from Leishmania spp. which, exists in two isoforms, the cytoplasmic PGKB and glycosomal PGKC differing in their biochemical properties. Computational analysis predicted the likelihood of a transmembrane helix only in the glycosomal isoform PGKC, of approximate length 20 residues in the 62-residue extension, ending at, arginine residues R471 and R472. From experimental studies using circular dichroism and NMR with deuterated sodium dodecyl sulfate, we find that the transmembrane helix spans residues 448 ± 2 to 476 in Leishmania mexicana PGKC. The significance of this observation is discussed in the context of glycosomal transport and substrate tunneling.
UR - http://www.scopus.com/inward/record.url?scp=84864280639&partnerID=8YFLogxK
U2 - 10.1016/j.molbiopara.2012.06.004
DO - 10.1016/j.molbiopara.2012.06.004
M3 - Article
C2 - 22710389
AN - SCOPUS:84864280639
SN - 0166-6851
VL - 185
SP - 27
EP - 35
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1
ER -