The zebrafish spi1 promoter drives myeloid-specific expression in stable transgenic fish

Alister C Ward, Dora McPhee, Melanie Condron, Sony Varma, Stephen H Cody, Sara M Onnebo, Barry H Paw, Leonard I Zon, Graham J Lieschke

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The spi1 (pu.1) gene has recently been identified as a useful marker of early myeloid cells in zebrafish. To enhance the versatility of this organism as a model for studying myeloid development, the promoter of this gene has been isolated and characterized. Transient transgenesis revealed that a 5.3 kilobase promoter fragment immediately upstream of the spi1 coding sequence was sufficient to drive expression of enhanced green fluorescent protein (EGFP) in injected embryos in a manner that largely recapitulated the native spi1 gene expression pattern. This fragment was successfully used to produce a germ line transgenic line of zebrafish with EGFP-expressing myeloid cells. These TG(spi1:EGFP)pA301 transgenic zebrafish represent a valuable tool for further studies of myeloid development and its perturbation.
Original languageEnglish
Pages (from-to)3238 - 3240
Number of pages3
Issue number9
Publication statusPublished - 2003

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