The WD40 Protein BamB Mediates Coupling of BAM Complexes into Assembly Precincts in the Bacterial Outer Membrane

Sachith D. Gunasinghe, Takuya Shiota, Christopher J. Stubenrauch, Keith E. Schulze, Chaille T. Webb, Alex J. Fulcher, Rhys A. Dunstan, Iain D. Hay, Thomas Naderer, Donna R. Whelan, Toby D.M. Bell, Kirstin D. Elgass, Richard A. Strugnell, Trevor Lithgow

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The β-barrel assembly machinery (BAM) complex is essential for localization of surface proteins on bacterial cells, but the mechanism by which it functions is unclear. We developed a direct stochastic optical reconstruction microscopy (dSTORM) methodology to view the BAM complex in situ. Single-cell analysis showed that discrete membrane precincts housing several BAM complexes are distributed across the E. coli surface, with a nearest neighbor distance of ∼200 nm. The auxiliary lipoprotein subunit BamB was crucial for this spatial distribution, and in situ crosslinking shows that BamB makes intimate contacts with BamA and BamB in neighboring BAM complexes within the precinct. The BAM complex precincts swell when outer membrane protein synthesis is maximal, visual proof that the precincts are active in protein assembly. This nanoscale interrogation of the BAM complex in situ suggests a model whereby bacterial outer membranes contain highly organized assembly precincts to drive integral protein assembly. Bacteria grow and divide by assembling new material into their surface membranes. Gunasinghe et al. used super-resolution microscopy and in situ crosslinking in live bacterial cells in order to visualize intimate contacts between BAM complexes suggesting a model whereby bacteria use highly organized precincts to drive membrane protein assembly.

Original languageEnglish
Pages (from-to)2782-2794
Number of pages13
JournalCell Reports
Volume23
Issue number9
DOIs
Publication statusPublished - 29 May 2018

Keywords

  • beta-barrel assembly
  • membrane domains
  • membrane proteins
  • membrane super-complexes
  • outer membrane biogenesis

Cite this

Gunasinghe, Sachith D. ; Shiota, Takuya ; Stubenrauch, Christopher J. ; Schulze, Keith E. ; Webb, Chaille T. ; Fulcher, Alex J. ; Dunstan, Rhys A. ; Hay, Iain D. ; Naderer, Thomas ; Whelan, Donna R. ; Bell, Toby D.M. ; Elgass, Kirstin D. ; Strugnell, Richard A. ; Lithgow, Trevor. / The WD40 Protein BamB Mediates Coupling of BAM Complexes into Assembly Precincts in the Bacterial Outer Membrane. In: Cell Reports. 2018 ; Vol. 23, No. 9. pp. 2782-2794.
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abstract = "The β-barrel assembly machinery (BAM) complex is essential for localization of surface proteins on bacterial cells, but the mechanism by which it functions is unclear. We developed a direct stochastic optical reconstruction microscopy (dSTORM) methodology to view the BAM complex in situ. Single-cell analysis showed that discrete membrane precincts housing several BAM complexes are distributed across the E. coli surface, with a nearest neighbor distance of ∼200 nm. The auxiliary lipoprotein subunit BamB was crucial for this spatial distribution, and in situ crosslinking shows that BamB makes intimate contacts with BamA and BamB in neighboring BAM complexes within the precinct. The BAM complex precincts swell when outer membrane protein synthesis is maximal, visual proof that the precincts are active in protein assembly. This nanoscale interrogation of the BAM complex in situ suggests a model whereby bacterial outer membranes contain highly organized assembly precincts to drive integral protein assembly. Bacteria grow and divide by assembling new material into their surface membranes. Gunasinghe et al. used super-resolution microscopy and in situ crosslinking in live bacterial cells in order to visualize intimate contacts between BAM complexes suggesting a model whereby bacteria use highly organized precincts to drive membrane protein assembly.",
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The WD40 Protein BamB Mediates Coupling of BAM Complexes into Assembly Precincts in the Bacterial Outer Membrane. / Gunasinghe, Sachith D.; Shiota, Takuya; Stubenrauch, Christopher J.; Schulze, Keith E.; Webb, Chaille T.; Fulcher, Alex J.; Dunstan, Rhys A.; Hay, Iain D.; Naderer, Thomas; Whelan, Donna R.; Bell, Toby D.M.; Elgass, Kirstin D.; Strugnell, Richard A.; Lithgow, Trevor.

In: Cell Reports, Vol. 23, No. 9, 29.05.2018, p. 2782-2794.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Gunasinghe, Sachith D.

AU - Shiota, Takuya

AU - Stubenrauch, Christopher J.

AU - Schulze, Keith E.

AU - Webb, Chaille T.

AU - Fulcher, Alex J.

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AU - Hay, Iain D.

AU - Naderer, Thomas

AU - Whelan, Donna R.

AU - Bell, Toby D.M.

AU - Elgass, Kirstin D.

AU - Strugnell, Richard A.

AU - Lithgow, Trevor

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N2 - The β-barrel assembly machinery (BAM) complex is essential for localization of surface proteins on bacterial cells, but the mechanism by which it functions is unclear. We developed a direct stochastic optical reconstruction microscopy (dSTORM) methodology to view the BAM complex in situ. Single-cell analysis showed that discrete membrane precincts housing several BAM complexes are distributed across the E. coli surface, with a nearest neighbor distance of ∼200 nm. The auxiliary lipoprotein subunit BamB was crucial for this spatial distribution, and in situ crosslinking shows that BamB makes intimate contacts with BamA and BamB in neighboring BAM complexes within the precinct. The BAM complex precincts swell when outer membrane protein synthesis is maximal, visual proof that the precincts are active in protein assembly. This nanoscale interrogation of the BAM complex in situ suggests a model whereby bacterial outer membranes contain highly organized assembly precincts to drive integral protein assembly. Bacteria grow and divide by assembling new material into their surface membranes. Gunasinghe et al. used super-resolution microscopy and in situ crosslinking in live bacterial cells in order to visualize intimate contacts between BAM complexes suggesting a model whereby bacteria use highly organized precincts to drive membrane protein assembly.

AB - The β-barrel assembly machinery (BAM) complex is essential for localization of surface proteins on bacterial cells, but the mechanism by which it functions is unclear. We developed a direct stochastic optical reconstruction microscopy (dSTORM) methodology to view the BAM complex in situ. Single-cell analysis showed that discrete membrane precincts housing several BAM complexes are distributed across the E. coli surface, with a nearest neighbor distance of ∼200 nm. The auxiliary lipoprotein subunit BamB was crucial for this spatial distribution, and in situ crosslinking shows that BamB makes intimate contacts with BamA and BamB in neighboring BAM complexes within the precinct. The BAM complex precincts swell when outer membrane protein synthesis is maximal, visual proof that the precincts are active in protein assembly. This nanoscale interrogation of the BAM complex in situ suggests a model whereby bacterial outer membranes contain highly organized assembly precincts to drive integral protein assembly. Bacteria grow and divide by assembling new material into their surface membranes. Gunasinghe et al. used super-resolution microscopy and in situ crosslinking in live bacterial cells in order to visualize intimate contacts between BAM complexes suggesting a model whereby bacteria use highly organized precincts to drive membrane protein assembly.

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