The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids

R. Brent Davey, Phillip I. Bird, Suzanne M. Nikoletti, Judyta Praszkier, James Pittard

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

The galactose operon of Escherichia coli K-12 has been used as a phenotypic marker for miniplasmids derived in vitro from R plasmids representing six incompatibility groups. This has enabled the development of a rapid incompatibility typing scheme in which the miniplasmids are used as incompatibility exemplars, their presence in strains being monitored on galactose fermentation indicator media.

Original languageEnglish
Pages (from-to)234-242
Number of pages9
JournalPlasmid
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Jan 1984
Externally publishedYes

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