The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array

Ji Hoon E. Joo, Ee Ming Wong, Laura Baglietto, Chol-Hee Jung, Helen Tsimiklis, Daniel J. Park, Nicholas C. Wong, Dallas R. English, John L. Hopper, Gianluca Severi, Graham G. Giles, Melissa C. Southey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.Results: In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.Conclusions: We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.

Original languageEnglish
Article number23
JournalBMC Biotechnology
Volume13
DOIs
Publication statusPublished - 15 Mar 2013
Externally publishedYes

Keywords

  • DNA methylation
  • Dried blood spots
  • Epigenetics
  • Genome-wide DNA methylation
  • Guthrie cards
  • Infinium HumanMethylation450

Cite this

Joo, Ji Hoon E. ; Wong, Ee Ming ; Baglietto, Laura ; Jung, Chol-Hee ; Tsimiklis, Helen ; Park, Daniel J. ; Wong, Nicholas C. ; English, Dallas R. ; Hopper, John L. ; Severi, Gianluca ; Giles, Graham G. ; Southey, Melissa C. / The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array. In: BMC Biotechnology. 2013 ; Vol. 13.
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title = "The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array",
abstract = "Background: Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.Results: In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.Conclusions: We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.",
keywords = "DNA methylation, Dried blood spots, Epigenetics, Genome-wide DNA methylation, Guthrie cards, Infinium HumanMethylation450",
author = "Joo, {Ji Hoon E.} and Wong, {Ee Ming} and Laura Baglietto and Chol-Hee Jung and Helen Tsimiklis and Park, {Daniel J.} and Wong, {Nicholas C.} and English, {Dallas R.} and Hopper, {John L.} and Gianluca Severi and Giles, {Graham G.} and Southey, {Melissa C.}",
year = "2013",
month = "3",
day = "15",
doi = "10.1186/1472-6750-13-23",
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Joo, JHE, Wong, EM, Baglietto, L, Jung, C-H, Tsimiklis, H, Park, DJ, Wong, NC, English, DR, Hopper, JL, Severi, G, Giles, GG & Southey, MC 2013, 'The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array' BMC Biotechnology, vol. 13, 23. https://doi.org/10.1186/1472-6750-13-23

The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array. / Joo, Ji Hoon E.; Wong, Ee Ming; Baglietto, Laura; Jung, Chol-Hee; Tsimiklis, Helen; Park, Daniel J.; Wong, Nicholas C.; English, Dallas R.; Hopper, John L.; Severi, Gianluca; Giles, Graham G.; Southey, Melissa C.

In: BMC Biotechnology, Vol. 13, 23, 15.03.2013.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array

AU - Joo, Ji Hoon E.

AU - Wong, Ee Ming

AU - Baglietto, Laura

AU - Jung, Chol-Hee

AU - Tsimiklis, Helen

AU - Park, Daniel J.

AU - Wong, Nicholas C.

AU - English, Dallas R.

AU - Hopper, John L.

AU - Severi, Gianluca

AU - Giles, Graham G.

AU - Southey, Melissa C.

PY - 2013/3/15

Y1 - 2013/3/15

N2 - Background: Dried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.Results: In this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.Conclusions: We, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.

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