The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans

Gregory M Davis; Shikui Tu; Joshua WT Anderson; Rhys N Colson; Menachem J Gunzburg; Michelle A. Francisco; Debashish Ray; Sean P Shrubsole; Julia A. Sobotka; Uri Seroussi; Robert X. Lao; Tuhin Maity; Monica Z. Wu; Katherine McJunkin; Quaid D. Morris; Timothy R. Hughes; Jacqueline A Wilce; Julie M. Claycomb; Zhiping Weng; Peter R. Boag

doi:10.7554/eLife.35478

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans

Gregory M Davis, Shikui Tu, Joshua WT Anderson, Rhys N Colson, Menachem J Gunzburg, Michelle A. Francisco, Debashish Ray, Sean P Shrubsole, Julia A. Sobotka, Uri Seroussi, Robert X. Lao, Tuhin Maity, Monica Z. Wu, Katherine McJunkin, Quaid D. Morris, Timothy R. Hughes, Jacqueline A Wilce, Julie M. Claycomb, Zhiping Weng, Peter R. Boag

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12 Citations (Scopus)

Abstract

Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

Original language	English
Article number	e35478
Number of pages	29
Journal	eLife
Volume	7
DOIs	https://doi.org/10.7554/eLife.35478
Publication status	Published - 21 Dec 2018

Keywords

22G-RNA
Caenorhabditis elegans
NHL-2
RNAi
TRIM-NHL

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Davis, G. M., Tu, S., Anderson, J. WT., Colson, R. N., Gunzburg, M. J., Francisco, M. A., Ray, D., Shrubsole, S. P., Sobotka, J. A., Seroussi, U., Lao, R. X., Maity, T., Wu, M. Z., McJunkin, K., Morris, Q. D., Hughes, T. R., Wilce, J. A., Claycomb, J. M., Weng, Z., & Boag, P. R. (2018). The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans. eLife, 7, Article e35478. https://doi.org/10.7554/eLife.35478

@article{4c99c85fdd58464c8c4c9612cc242b94,

title = "The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans",

abstract = "Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.",

keywords = "22G-RNA, Caenorhabditis elegans, NHL-2, RNAi, TRIM-NHL",

author = "Davis, {Gregory M} and Shikui Tu and Anderson, {Joshua WT} and Colson, {Rhys N} and Gunzburg, {Menachem J} and Francisco, {Michelle A.} and Debashish Ray and Shrubsole, {Sean P} and Sobotka, {Julia A.} and Uri Seroussi and Lao, {Robert X.} and Tuhin Maity and Wu, {Monica Z.} and Katherine McJunkin and Morris, {Quaid D.} and Hughes, {Timothy R.} and Wilce, {Jacqueline A} and Claycomb, {Julie M.} and Zhiping Weng and Boag, {Peter R.}",

year = "2018",

month = dec,

day = "21",

doi = "10.7554/eLife.35478",

language = "English",

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Davis, GM, Tu, S, Anderson, JWT, Colson, RN, Gunzburg, MJ, Francisco, MA, Ray, D, Shrubsole, SP, Sobotka, JA, Seroussi, U, Lao, RX, Maity, T, Wu, MZ, McJunkin, K, Morris, QD, Hughes, TR, Wilce, JA, Claycomb, JM, Weng, Z & Boag, PR 2018, 'The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans', eLife, vol. 7, e35478. https://doi.org/10.7554/eLife.35478

TY - JOUR

T1 - The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans

AU - Davis, Gregory M

AU - Tu, Shikui

AU - Anderson, Joshua WT

AU - Colson, Rhys N

AU - Gunzburg, Menachem J

AU - Francisco, Michelle A.

AU - Ray, Debashish

AU - Shrubsole, Sean P

AU - Sobotka, Julia A.

AU - Seroussi, Uri

AU - Lao, Robert X.

AU - Maity, Tuhin

AU - Wu, Monica Z.

AU - McJunkin, Katherine

AU - Morris, Quaid D.

AU - Hughes, Timothy R.

AU - Wilce, Jacqueline A

AU - Claycomb, Julie M.

AU - Weng, Zhiping

AU - Boag, Peter R.

PY - 2018/12/21

Y1 - 2018/12/21

N2 - Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

AB - Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

KW - 22G-RNA

KW - Caenorhabditis elegans

KW - NHL-2

KW - RNAi

KW - TRIM-NHL

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U2 - 10.7554/eLife.35478

DO - 10.7554/eLife.35478

M3 - Article

C2 - 30575518

AN - SCOPUS:85060567557

SN - 2050-084X

VL - 7

JO - eLife

JF - eLife

M1 - e35478

ER -

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans

Abstract

Keywords

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