The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans

Gregory M Davis, Shikui Tu, Joshua WT Anderson, Rhys N Colson, Menachem J Gunzburg, Michelle A. Francisco, Debashish Ray, Sean P Shrubsole, Julia A. Sobotka, Uri Seroussi, Robert X. Lao, Tuhin Maity, Monica Z. Wu, Katherine McJunkin, Quaid D. Morris, Timothy R. Hughes, Jacqueline A Wilce, Julie M. Claycomb, Zhiping Weng, Peter R. Boag

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

Original languageEnglish
Article numbere35478
Number of pages29
JournaleLife
Volume7
DOIs
Publication statusPublished - 21 Dec 2018

Keywords

  • 22G-RNA
  • Caenorhabditis elegans
  • NHL-2
  • RNAi
  • TRIM-NHL

Cite this

Davis, Gregory M ; Tu, Shikui ; Anderson, Joshua WT ; Colson, Rhys N ; Gunzburg, Menachem J ; Francisco, Michelle A. ; Ray, Debashish ; Shrubsole, Sean P ; Sobotka, Julia A. ; Seroussi, Uri ; Lao, Robert X. ; Maity, Tuhin ; Wu, Monica Z. ; McJunkin, Katherine ; Morris, Quaid D. ; Hughes, Timothy R. ; Wilce, Jacqueline A ; Claycomb, Julie M. ; Weng, Zhiping ; Boag, Peter R. / The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans. In: eLife. 2018 ; Vol. 7.
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title = "The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans",
abstract = "Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.",
keywords = "22G-RNA, Caenorhabditis elegans, NHL-2, RNAi, TRIM-NHL",
author = "Davis, {Gregory M} and Shikui Tu and Anderson, {Joshua WT} and Colson, {Rhys N} and Gunzburg, {Menachem J} and Francisco, {Michelle A.} and Debashish Ray and Shrubsole, {Sean P} and Sobotka, {Julia A.} and Uri Seroussi and Lao, {Robert X.} and Tuhin Maity and Wu, {Monica Z.} and Katherine McJunkin and Morris, {Quaid D.} and Hughes, {Timothy R.} and Wilce, {Jacqueline A} and Claycomb, {Julie M.} and Zhiping Weng and Boag, {Peter R.}",
year = "2018",
month = "12",
day = "21",
doi = "10.7554/eLife.35478",
language = "English",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications, Ltd",

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Davis, GM, Tu, S, Anderson, JWT, Colson, RN, Gunzburg, MJ, Francisco, MA, Ray, D, Shrubsole, SP, Sobotka, JA, Seroussi, U, Lao, RX, Maity, T, Wu, MZ, McJunkin, K, Morris, QD, Hughes, TR, Wilce, JA, Claycomb, JM, Weng, Z & Boag, PR 2018, 'The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans' eLife, vol. 7, e35478. https://doi.org/10.7554/eLife.35478

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans. / Davis, Gregory M; Tu, Shikui; Anderson, Joshua WT; Colson, Rhys N; Gunzburg, Menachem J; Francisco, Michelle A.; Ray, Debashish; Shrubsole, Sean P; Sobotka, Julia A.; Seroussi, Uri; Lao, Robert X.; Maity, Tuhin; Wu, Monica Z.; McJunkin, Katherine; Morris, Quaid D.; Hughes, Timothy R.; Wilce, Jacqueline A; Claycomb, Julie M.; Weng, Zhiping; Boag, Peter R.

In: eLife, Vol. 7, e35478, 21.12.2018.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. Elegans

AU - Davis, Gregory M

AU - Tu, Shikui

AU - Anderson, Joshua WT

AU - Colson, Rhys N

AU - Gunzburg, Menachem J

AU - Francisco, Michelle A.

AU - Ray, Debashish

AU - Shrubsole, Sean P

AU - Sobotka, Julia A.

AU - Seroussi, Uri

AU - Lao, Robert X.

AU - Maity, Tuhin

AU - Wu, Monica Z.

AU - McJunkin, Katherine

AU - Morris, Quaid D.

AU - Hughes, Timothy R.

AU - Wilce, Jacqueline A

AU - Claycomb, Julie M.

AU - Weng, Zhiping

AU - Boag, Peter R.

PY - 2018/12/21

Y1 - 2018/12/21

N2 - Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

AB - Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

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KW - Caenorhabditis elegans

KW - NHL-2

KW - RNAi

KW - TRIM-NHL

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U2 - 10.7554/eLife.35478

DO - 10.7554/eLife.35478

M3 - Article

VL - 7

JO - eLife

JF - eLife

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