The TAFs of TFIID bind and rearrange the topology of the TATA-less RPS5 promoter

Sarah N. Le, Christopher R. Brown, Stacy Harvey, Hinrich Boeger, Hans Elmlund, Dominika Elmlund

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The general transcription factor TFIID is a core promoter selectivity factor that recognizes DNA sequence elements and nucleates the assembly of a pre-initiation complex (PIC). The mechanism by which TFIID recognizes the promoter is poorly understood. The TATA-box binding protein (TBP) is a subunit of the multi-protein TFIID complex believed to be key in this process. We reconstituted transcription from highly purified components on a ribosomal protein gene (RPS5) and discovered that TFIID∆TBP binds and rearranges the promoter DNA topology independent of TBP. TFIID∆TBP binds ~200 bp of the promoter and changes the DNA topology to a larger extent than the nucleosome core particle. We show that TBP inhibits the DNA binding activities of TFIID∆TBP and conclude that the complete TFIID complex may represent an auto-inhibited state. Furthermore, we show that the DNA binding activities of TFIID∆TBP are required for assembly of a PIC poised to select the correct transcription start site (TSS).

Original languageEnglish
Article number3290
Number of pages12
JournalInternational Journal of Molecular Sciences
Volume20
Issue number13
DOIs
Publication statusPublished - 4 Jul 2019

Keywords

  • DNA topology
  • Housekeeping gene transcription
  • Ribosomal protein 5
  • TAF complex
  • TBP-associated factor

Cite this

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title = "The TAFs of TFIID bind and rearrange the topology of the TATA-less RPS5 promoter",
abstract = "The general transcription factor TFIID is a core promoter selectivity factor that recognizes DNA sequence elements and nucleates the assembly of a pre-initiation complex (PIC). The mechanism by which TFIID recognizes the promoter is poorly understood. The TATA-box binding protein (TBP) is a subunit of the multi-protein TFIID complex believed to be key in this process. We reconstituted transcription from highly purified components on a ribosomal protein gene (RPS5) and discovered that TFIID∆TBP binds and rearranges the promoter DNA topology independent of TBP. TFIID∆TBP binds ~200 bp of the promoter and changes the DNA topology to a larger extent than the nucleosome core particle. We show that TBP inhibits the DNA binding activities of TFIID∆TBP and conclude that the complete TFIID complex may represent an auto-inhibited state. Furthermore, we show that the DNA binding activities of TFIID∆TBP are required for assembly of a PIC poised to select the correct transcription start site (TSS).",
keywords = "DNA topology, Housekeeping gene transcription, Ribosomal protein 5, TAF complex, TBP-associated factor",
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The TAFs of TFIID bind and rearrange the topology of the TATA-less RPS5 promoter. / Le, Sarah N.; Brown, Christopher R.; Harvey, Stacy; Boeger, Hinrich; Elmlund, Hans; Elmlund, Dominika.

In: International Journal of Molecular Sciences, Vol. 20, No. 13, 3290, 04.07.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Le, Sarah N.

AU - Brown, Christopher R.

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AU - Elmlund, Hans

AU - Elmlund, Dominika

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AB - The general transcription factor TFIID is a core promoter selectivity factor that recognizes DNA sequence elements and nucleates the assembly of a pre-initiation complex (PIC). The mechanism by which TFIID recognizes the promoter is poorly understood. The TATA-box binding protein (TBP) is a subunit of the multi-protein TFIID complex believed to be key in this process. We reconstituted transcription from highly purified components on a ribosomal protein gene (RPS5) and discovered that TFIID∆TBP binds and rearranges the promoter DNA topology independent of TBP. TFIID∆TBP binds ~200 bp of the promoter and changes the DNA topology to a larger extent than the nucleosome core particle. We show that TBP inhibits the DNA binding activities of TFIID∆TBP and conclude that the complete TFIID complex may represent an auto-inhibited state. Furthermore, we show that the DNA binding activities of TFIID∆TBP are required for assembly of a PIC poised to select the correct transcription start site (TSS).

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