Abstract
The general transcription factor TFIID is a core promoter selectivity factor that recognizes DNA sequence elements and nucleates the assembly of a pre-initiation complex (PIC). The mechanism by which TFIID recognizes the promoter is poorly understood. The TATA-box binding protein (TBP) is a subunit of the multi-protein TFIID complex believed to be key in this process. We reconstituted transcription from highly purified components on a ribosomal protein gene (RPS5) and discovered that TFIID∆TBP binds and rearranges the promoter DNA topology independent of TBP. TFIID∆TBP binds ~200 bp of the promoter and changes the DNA topology to a larger extent than the nucleosome core particle. We show that TBP inhibits the DNA binding activities of TFIID∆TBP and conclude that the complete TFIID complex may represent an auto-inhibited state. Furthermore, we show that the DNA binding activities of TFIID∆TBP are required for assembly of a PIC poised to select the correct transcription start site (TSS).
| Original language | English |
|---|---|
| Article number | 3290 |
| Number of pages | 12 |
| Journal | International Journal of Molecular Sciences |
| Volume | 20 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 4 Jul 2019 |
Keywords
- DNA topology
- Housekeeping gene transcription
- Ribosomal protein 5
- TAF complex
- TBP-associated factor
Cite this
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The TAFs of TFIID bind and rearrange the topology of the TATA-less RPS5 promoter. / Le, Sarah N.; Brown, Christopher R.; Harvey, Stacy; Boeger, Hinrich; Elmlund, Hans; Elmlund, Dominika.
In: International Journal of Molecular Sciences, Vol. 20, No. 13, 3290, 04.07.2019.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - The TAFs of TFIID bind and rearrange the topology of the TATA-less RPS5 promoter
AU - Le, Sarah N.
AU - Brown, Christopher R.
AU - Harvey, Stacy
AU - Boeger, Hinrich
AU - Elmlund, Hans
AU - Elmlund, Dominika
PY - 2019/7/4
Y1 - 2019/7/4
N2 - The general transcription factor TFIID is a core promoter selectivity factor that recognizes DNA sequence elements and nucleates the assembly of a pre-initiation complex (PIC). The mechanism by which TFIID recognizes the promoter is poorly understood. The TATA-box binding protein (TBP) is a subunit of the multi-protein TFIID complex believed to be key in this process. We reconstituted transcription from highly purified components on a ribosomal protein gene (RPS5) and discovered that TFIID∆TBP binds and rearranges the promoter DNA topology independent of TBP. TFIID∆TBP binds ~200 bp of the promoter and changes the DNA topology to a larger extent than the nucleosome core particle. We show that TBP inhibits the DNA binding activities of TFIID∆TBP and conclude that the complete TFIID complex may represent an auto-inhibited state. Furthermore, we show that the DNA binding activities of TFIID∆TBP are required for assembly of a PIC poised to select the correct transcription start site (TSS).
AB - The general transcription factor TFIID is a core promoter selectivity factor that recognizes DNA sequence elements and nucleates the assembly of a pre-initiation complex (PIC). The mechanism by which TFIID recognizes the promoter is poorly understood. The TATA-box binding protein (TBP) is a subunit of the multi-protein TFIID complex believed to be key in this process. We reconstituted transcription from highly purified components on a ribosomal protein gene (RPS5) and discovered that TFIID∆TBP binds and rearranges the promoter DNA topology independent of TBP. TFIID∆TBP binds ~200 bp of the promoter and changes the DNA topology to a larger extent than the nucleosome core particle. We show that TBP inhibits the DNA binding activities of TFIID∆TBP and conclude that the complete TFIID complex may represent an auto-inhibited state. Furthermore, we show that the DNA binding activities of TFIID∆TBP are required for assembly of a PIC poised to select the correct transcription start site (TSS).
KW - DNA topology
KW - Housekeeping gene transcription
KW - Ribosomal protein 5
KW - TAF complex
KW - TBP-associated factor
UR - http://www.scopus.com/inward/record.url?scp=85069269373&partnerID=8YFLogxK
U2 - 10.3390/ijms20133290
DO - 10.3390/ijms20133290
M3 - Article
C2 - 31277458
AN - SCOPUS:85069269373
VL - 20
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 13
M1 - 3290
ER -