TY - JOUR
T1 - The synergistic treatment of cyclolinopeptide J and calcium carbonate nanoparticles for osteoporosis via BMP/Wnt signaling
T2 - in vivo and in vitro
AU - Chen, Jiazi
AU - Li, Wen
AU - Lee, Yee Ying
AU - Cai, Zizhe
AU - Chen, Jing
AU - Wang, Yong
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (31920103012), and the National Natural Science Foundation of China (32201933).
Publisher Copyright:
© 2023 The Authors
PY - 2023/11
Y1 - 2023/11
N2 - This research focuses on the investigation of cyclolinopeptide J (CLJ), a bioactive peptide naturally present in flaxseed, which loaded in porous calcium carbonate (CA) nanoparticles (JCA) to augment the effectiveness of CLJ on osteogenesis. The JCA was successfully synthesized with a high loading capacity (47.8 %) and encapsulation efficiency (95.6 %). Results showed that CLJ exerted an excellent osteogenic effect at 10 µM in MC3T3-E1 cells. CLJ and CA have been shown to activate osteogenic factors by modulating the Wnt/β-catenin and BMP/Smad signaling pathways. Furthermore, JCA treatment exhibited a remarkable ability to restore the intricate trabecular characteristics of bone in OVX-induced mice. The trabecular bone architecture observed in JCA-treated mice closely resembled that of healthy controls, indicating a substantial amelioration of osteoporotic bone loss. Our findings highlight the synergistic treatment of CLJ and CA in restoring bone integrity and structure and provide compelling evidence for the effectiveness of this novel functional supplement.
AB - This research focuses on the investigation of cyclolinopeptide J (CLJ), a bioactive peptide naturally present in flaxseed, which loaded in porous calcium carbonate (CA) nanoparticles (JCA) to augment the effectiveness of CLJ on osteogenesis. The JCA was successfully synthesized with a high loading capacity (47.8 %) and encapsulation efficiency (95.6 %). Results showed that CLJ exerted an excellent osteogenic effect at 10 µM in MC3T3-E1 cells. CLJ and CA have been shown to activate osteogenic factors by modulating the Wnt/β-catenin and BMP/Smad signaling pathways. Furthermore, JCA treatment exhibited a remarkable ability to restore the intricate trabecular characteristics of bone in OVX-induced mice. The trabecular bone architecture observed in JCA-treated mice closely resembled that of healthy controls, indicating a substantial amelioration of osteoporotic bone loss. Our findings highlight the synergistic treatment of CLJ and CA in restoring bone integrity and structure and provide compelling evidence for the effectiveness of this novel functional supplement.
KW - BMP/Wnt signaling
KW - Calcium carbonate nanoparticles
KW - Cyclolinopeptide J
KW - Osteoporosis
KW - Synergistic treatment
UR - http://www.scopus.com/inward/record.url?scp=85173968498&partnerID=8YFLogxK
U2 - 10.1016/j.jff.2023.105826
DO - 10.1016/j.jff.2023.105826
M3 - Article
AN - SCOPUS:85173968498
SN - 1756-4646
VL - 110
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 105826
ER -