The synergistic treatment of cyclolinopeptide J and calcium carbonate nanoparticles for osteoporosis via BMP/Wnt signaling: in vivo and in vitro

Jiazi Chen, Wen Li, Yee Ying Lee, Zizhe Cai, Jing Chen, Yong Wang

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)


This research focuses on the investigation of cyclolinopeptide J (CLJ), a bioactive peptide naturally present in flaxseed, which loaded in porous calcium carbonate (CA) nanoparticles (JCA) to augment the effectiveness of CLJ on osteogenesis. The JCA was successfully synthesized with a high loading capacity (47.8 %) and encapsulation efficiency (95.6 %). Results showed that CLJ exerted an excellent osteogenic effect at 10 µM in MC3T3-E1 cells. CLJ and CA have been shown to activate osteogenic factors by modulating the Wnt/β-catenin and BMP/Smad signaling pathways. Furthermore, JCA treatment exhibited a remarkable ability to restore the intricate trabecular characteristics of bone in OVX-induced mice. The trabecular bone architecture observed in JCA-treated mice closely resembled that of healthy controls, indicating a substantial amelioration of osteoporotic bone loss. Our findings highlight the synergistic treatment of CLJ and CA in restoring bone integrity and structure and provide compelling evidence for the effectiveness of this novel functional supplement.

Original languageEnglish
Article number105826
Number of pages12
JournalJournal of Functional Foods
Publication statusPublished - Nov 2023


  • BMP/Wnt signaling
  • Calcium carbonate nanoparticles
  • Cyclolinopeptide J
  • Osteoporosis
  • Synergistic treatment

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