TY - JOUR
T1 - The simultaneous determination of 3,4-dihydroxyphenylethylene glycol, 3,4-dihydroxyphenylacetic acid and catecholamines in brain tissue by high performance liquid chromatography with electrochemical detection
AU - Howes, L. G.
AU - Summers, R. J.
AU - Rowe, P. R.
AU - Loui, W. J.
PY - 1983/8/8
Y1 - 1983/8/8
N2 - An assay is described for the simultaneous determination of the deaminated metabolites 3,4-dihydroxyphenylethylene glycol (DHPG) and 3,4-dihydroxyphenylacetic acid (DOPAC), together with noradrenaline, dopamine and adrenaline in brain using high performance liquid chromatography with electrochemical detection. The procedure is simple, specific, and has a limit of detection for each catechol of 5 pg. It offers the advantage of measuring the major monoamine oxidase metabolites of both noradrenaline (i.e. DHPG) and dopamine (i.e. DOPAC), which in some circumstances may reflect neuronal activity, simultaneously with their catecholamine neurotransmitter precursors.
AB - An assay is described for the simultaneous determination of the deaminated metabolites 3,4-dihydroxyphenylethylene glycol (DHPG) and 3,4-dihydroxyphenylacetic acid (DOPAC), together with noradrenaline, dopamine and adrenaline in brain using high performance liquid chromatography with electrochemical detection. The procedure is simple, specific, and has a limit of detection for each catechol of 5 pg. It offers the advantage of measuring the major monoamine oxidase metabolites of both noradrenaline (i.e. DHPG) and dopamine (i.e. DOPAC), which in some circumstances may reflect neuronal activity, simultaneously with their catecholamine neurotransmitter precursors.
KW - 3,4-dihydroxyphenylacetic acid
KW - 3,4-dihydroxyphenylethylene glycol
KW - adrenaline
KW - dopamine
KW - electrochemical detection
KW - high performance liquid chromatography
KW - noradrenaline
KW - rat brain
UR - http://www.scopus.com/inward/record.url?scp=0021097732&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(83)90390-7
DO - 10.1016/0304-3940(83)90390-7
M3 - Article
C2 - 6415553
AN - SCOPUS:0021097732
SN - 0304-3940
VL - 38
SP - 327
EP - 332
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -