The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum

Christine L Knox, Samantha J Dando, Ilias Nitsos, Suhas G Kallapur, Alan H Jobe, Diane Payton, Timothy James Murugesan Moss, John P Newnham

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid from asymptomatic pregnancies and placental infections. U. parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects of chronic ureaplasma infection of amniotic fluid on the fetus and chorioamnion are dependent on the serovar, the dose, and variation of the ureaplasma multiple banded antigen (MBA) and mba gene. We injected high or low dose U. parvum serovar 3, serovar 6 (or vehicle) intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by Cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005); and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (/=9 mba variants showed no/little inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes.
Original languageEnglish
Pages (from-to)415 - 426
Number of pages12
JournalBiology of Reproduction
Volume83
Issue number3
DOIs
Publication statusPublished - 2010

Cite this

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title = "The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum",
abstract = "Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid from asymptomatic pregnancies and placental infections. U. parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects of chronic ureaplasma infection of amniotic fluid on the fetus and chorioamnion are dependent on the serovar, the dose, and variation of the ureaplasma multiple banded antigen (MBA) and mba gene. We injected high or low dose U. parvum serovar 3, serovar 6 (or vehicle) intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by Cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005); and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (/=9 mba variants showed no/little inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes.",
author = "Knox, {Christine L} and Dando, {Samantha J} and Ilias Nitsos and Kallapur, {Suhas G} and Jobe, {Alan H} and Diane Payton and Moss, {Timothy James Murugesan} and Newnham, {John P}",
year = "2010",
doi = "10.1095/biolreprod.109.083121",
language = "English",
volume = "83",
pages = "415 -- 426",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "3",

}

The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum. / Knox, Christine L; Dando, Samantha J; Nitsos, Ilias; Kallapur, Suhas G; Jobe, Alan H; Payton, Diane; Moss, Timothy James Murugesan; Newnham, John P.

In: Biology of Reproduction, Vol. 83, No. 3, 2010, p. 415 - 426.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The severity of chorioamnionitis in pregnant sheep is associated with in vivo variation of the surface-exposed multiple-banded antigen/gene of Ureaplasma parvum

AU - Knox, Christine L

AU - Dando, Samantha J

AU - Nitsos, Ilias

AU - Kallapur, Suhas G

AU - Jobe, Alan H

AU - Payton, Diane

AU - Moss, Timothy James Murugesan

AU - Newnham, John P

PY - 2010

Y1 - 2010

N2 - Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid from asymptomatic pregnancies and placental infections. U. parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects of chronic ureaplasma infection of amniotic fluid on the fetus and chorioamnion are dependent on the serovar, the dose, and variation of the ureaplasma multiple banded antigen (MBA) and mba gene. We injected high or low dose U. parvum serovar 3, serovar 6 (or vehicle) intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by Cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005); and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (/=9 mba variants showed no/little inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes.

AB - Ureaplasma species are the bacteria most frequently isolated from human amniotic fluid from asymptomatic pregnancies and placental infections. U. parvum serovars 3 and 6 are the most prevalent serovars isolated from men and women. We hypothesized that the effects of chronic ureaplasma infection of amniotic fluid on the fetus and chorioamnion are dependent on the serovar, the dose, and variation of the ureaplasma multiple banded antigen (MBA) and mba gene. We injected high or low dose U. parvum serovar 3, serovar 6 (or vehicle) intra-amniotically into pregnant ewes at 55 days of gestation (term = 150 days) and examined the chorioamnion, amniotic fluid, and fetal lung tissue of animals delivered by Cesarean section at 125 days of gestation. Variation of the multiple banded antigen/mba generated by serovar 3 and serovar 6 ureaplasmas in vivo were compared by PCR assay and western blot. Ureaplasma inoculums demonstrated only one (serovar 3) or two (serovar 6) MBA variants in vitro, but numerous antigenic variants were generated in vivo: serovar 6 passage 1 amniotic fluid cultures contained more MBA size variants than serovar 3 (P = 0.005); and ureaplasma titers were inversely related to the number of variants (P = 0.025). The severity of chorioamnionitis varied between animals. Low numbers of mba size variants (/=9 mba variants showed no/little inflammation. These differences in chorioamnion inflammation may explain why not all women with in utero Ureaplasma spp. experience adverse pregnancy outcomes.

UR - http://www.biolreprod.org/content/83/3/415.full.pdf+html

U2 - 10.1095/biolreprod.109.083121

DO - 10.1095/biolreprod.109.083121

M3 - Article

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EP - 426

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 3

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