The scottish structural proteomics facility: Targets, methods and outputs

Muse Oke, Lester G. Carter, Kenneth A. Johnson, Huanting Liu, Stephen A. McMahon, Xuan Yan, Melina Kerou, Nadine D. Weikart, Nadia F. Kadi, Md Arif Sheikh, Stefan Schmelz, Mark Dorward, Michal Zawadzki, Christopher Cozens, Helen Falconer, Helen Powers, Ian M. Overton, C. A Johannes Van Niekerk, Xu Peng, Prakash PatelRoger A. Garrett, David Prangishvili, Catherine H. Botting, Peter J. Coote, David T F Dryden, Geoffrey J Barton, Ulrich Schwarz-Linek, Gregory L. Challis, Garry L Taylor, Malcolm F. White, James H. Naismith

Research output: Contribution to journalArticleResearchpeer-review

83 Citations (Scopus)

Abstract

The Scottish Structural Proteomics Facility was funded to develop a laboratory scale approach to high throughput structure determination. The effort was successful in that over 40 structures were determined. These structures and the methods harnessed to obtain them are reported here. This report reflects on the value of automation but also on the continued requirement for a high degree of scientific and technical expertise. The efficiency of the process poses challenges to the current paradigm of structural analysis and publication. In the 5 year period we published ten peer-reviewed papers reporting structural data arising from the pipeline. Nevertheless, the number of structures solved exceeded our ability to analyse and publish each new finding. By reporting the experimental details and depositing the structures we hope to maximize the impact of the project by allowing others to follow up the relevant biology.

Original languageEnglish
Pages (from-to)167-180
Number of pages14
JournalJournal of Structural and Functional Genomics
Volume11
Issue number2
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

Keywords

  • High-throughput
  • Protein crystallography
  • SSPF
  • Structural proteomics

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