The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida

B Helen H Jost, Preecha Homchampa, Richard Strugnell, Ben Adler

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The use of theBacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains ofPasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that thesacB gene can not be used as a positive selection system inP. multocida.
Original languageEnglish
Pages (from-to)189 - 191
Number of pages3
JournalMolecular Biotechnology
Issue number2
Publication statusPublished - 1997

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