The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida

B Helen H Jost; Preecha Homchampa; Richard Strugnell; Ben Adler

The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida

B Helen H Jost, Preecha Homchampa, Richard Strugnell, Ben Adler

Microbiology

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

The use of theBacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains ofPasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that thesacB gene can not be used as a positive selection system inP. multocida.

Original language	English
Pages (from-to)	189 - 191
Number of pages	3
Journal	Molecular Biotechnology
Volume	8
Issue number	2
Publication status	Published - 1997

Cite this

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title = "The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida",

abstract = "The use of theBacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains ofPasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that thesacB gene can not be used as a positive selection system inP. multocida.",

author = "Jost, {B Helen H} and Preecha Homchampa and Richard Strugnell and Ben Adler",

year = "1997",

language = "English",

volume = "8",

pages = "189 -- 191",

journal = "Molecular Biotechnology",

issn = "1073-6085",

publisher = "Springer",

number = "2",

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TY - JOUR

T1 - The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida

AU - Jost, B Helen H

AU - Homchampa, Preecha

AU - Strugnell, Richard

AU - Adler, Ben

PY - 1997

Y1 - 1997

N2 - The use of theBacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains ofPasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that thesacB gene can not be used as a positive selection system inP. multocida.

AB - The use of theBacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains ofPasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that thesacB gene can not be used as a positive selection system inP. multocida.

M3 - Article

SN - 1073-6085

VL - 8

SP - 189

EP - 191

JO - Molecular Biotechnology

JF - Molecular Biotechnology

IS - 2

ER -