The role of WDR5 in silencing human fetal globin gene expression

Zhen Xu, Yinghong He, Junyi Ju, Gerhard Rank, Loretta Cerruti, Chi Ma, Richard J Simpson, Robert L Moritz, Stephen Marsden Jane, Quan Zhao

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Background: Histone H3 lysine 4 (K4) methylation has been linked with transcriptional activity in mammalian cells. The WD40-repeat protein, WDR5, is an essential component of the MLL complex that induces histone H3 K4 methylation, but the role of WDR5 in human globin gene regulation has not yet been established. Design and Methods : To study the role of WDR5 in human globin gene regulation, we performed knockdown experiments in both K562 cells and primary human bone marrow erythroid progenitor cells (BMC). The effects of WDR5 knockdown on ?-globin gene expression were determined. Biochemical approaches were also employed to investigate WDR5 interaction molecules. Chromosomal marks in the globin locus were analyzed by ChIP.
Original languageEnglish
Pages (from-to)1632 - 1640
Number of pages9
JournalHaematologica: the hematology journal
Volume97
Issue number11
DOIs
Publication statusPublished - 2012

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