The role of WDR5 in silencing human fetal globin gene expression

Zhen Xu; Yinghong He; Junyi Ju; Gerhard Rank; Loretta Cerruti; Chi Ma; Richard J Simpson; Robert L Moritz; Stephen Marsden Jane; Quan Zhao

doi:10.3324/haematol.2012.061937

The role of WDR5 in silencing human fetal globin gene expression

Zhen Xu, Yinghong He, Junyi Ju, Gerhard Rank, Loretta Cerruti, Chi Ma, Richard J Simpson, Robert L Moritz, Stephen Marsden Jane, Quan Zhao

Medicine Alfred Hospital

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

Background: Histone H3 lysine 4 (K4) methylation has been linked with transcriptional activity in mammalian cells. The WD40-repeat protein, WDR5, is an essential component of the MLL complex that induces histone H3 K4 methylation, but the role of WDR5 in human globin gene regulation has not yet been established. Design and Methods : To study the role of WDR5 in human globin gene regulation, we performed knockdown experiments in both K562 cells and primary human bone marrow erythroid progenitor cells (BMC). The effects of WDR5 knockdown on ?-globin gene expression were determined. Biochemical approaches were also employed to investigate WDR5 interaction molecules. Chromosomal marks in the globin locus were analyzed by ChIP.

Original language	English
Pages (from-to)	1632 - 1640
Number of pages	9
Journal	Haematologica
Volume	97
Issue number	11
DOIs	https://doi.org/10.3324/haematol.2012.061937
Publication status	Published - 2012

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10.3324/haematol.2012.061937

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title = "The role of WDR5 in silencing human fetal globin gene expression",

abstract = "Background: Histone H3 lysine 4 (K4) methylation has been linked with transcriptional activity in mammalian cells. The WD40-repeat protein, WDR5, is an essential component of the MLL complex that induces histone H3 K4 methylation, but the role of WDR5 in human globin gene regulation has not yet been established. Design and Methods : To study the role of WDR5 in human globin gene regulation, we performed knockdown experiments in both K562 cells and primary human bone marrow erythroid progenitor cells (BMC). The effects of WDR5 knockdown on ?-globin gene expression were determined. Biochemical approaches were also employed to investigate WDR5 interaction molecules. Chromosomal marks in the globin locus were analyzed by ChIP.",

author = "Zhen Xu and Yinghong He and Junyi Ju and Gerhard Rank and Loretta Cerruti and Chi Ma and Simpson, {Richard J} and Moritz, {Robert L} and Jane, {Stephen Marsden} and Quan Zhao",

year = "2012",

doi = "10.3324/haematol.2012.061937",

language = "English",

volume = "97",

pages = "1632 -- 1640",

journal = "Haematologica",

issn = "0390-6078",

publisher = "Fondazione Ferrata Storti",

number = "11",

}

TY - JOUR

T1 - The role of WDR5 in silencing human fetal globin gene expression

AU - Xu, Zhen

AU - He, Yinghong

AU - Ju, Junyi

AU - Rank, Gerhard

AU - Cerruti, Loretta

AU - Ma, Chi

AU - Simpson, Richard J

AU - Moritz, Robert L

AU - Jane, Stephen Marsden

AU - Zhao, Quan

PY - 2012

Y1 - 2012

N2 - Background: Histone H3 lysine 4 (K4) methylation has been linked with transcriptional activity in mammalian cells. The WD40-repeat protein, WDR5, is an essential component of the MLL complex that induces histone H3 K4 methylation, but the role of WDR5 in human globin gene regulation has not yet been established. Design and Methods : To study the role of WDR5 in human globin gene regulation, we performed knockdown experiments in both K562 cells and primary human bone marrow erythroid progenitor cells (BMC). The effects of WDR5 knockdown on ?-globin gene expression were determined. Biochemical approaches were also employed to investigate WDR5 interaction molecules. Chromosomal marks in the globin locus were analyzed by ChIP.

AB - Background: Histone H3 lysine 4 (K4) methylation has been linked with transcriptional activity in mammalian cells. The WD40-repeat protein, WDR5, is an essential component of the MLL complex that induces histone H3 K4 methylation, but the role of WDR5 in human globin gene regulation has not yet been established. Design and Methods : To study the role of WDR5 in human globin gene regulation, we performed knockdown experiments in both K562 cells and primary human bone marrow erythroid progenitor cells (BMC). The effects of WDR5 knockdown on ?-globin gene expression were determined. Biochemical approaches were also employed to investigate WDR5 interaction molecules. Chromosomal marks in the globin locus were analyzed by ChIP.

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487434/pdf/0971632.pdf

U2 - 10.3324/haematol.2012.061937

DO - 10.3324/haematol.2012.061937

M3 - Article

VL - 97

SP - 1632

EP - 1640

JO - Haematologica

JF - Haematologica

SN - 0390-6078

IS - 11

ER -

The role of WDR5 in silencing human fetal globin gene expression

Abstract

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