The role of vitamin D on circulating memory T cells in children

The Generation R study

Kirsten I.M. Looman, Michelle A.E. Jansen, Trudy Voortman, Diana van den Heuvel, Vincent W.V. Jaddoe, Oscar H. Franco, Menno C. van Zelm, Henriëtte A. Moll

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Background: Previous studies have demonstrated that vitamin D affects T-cell function and maturation via the vitamin D receptor. However, no studies in children have been performed on this topic. Because most of the T-cell memory is formed in the first 5 years of life, we aimed to determine the association between serum 25-hydroxyvitamin D (25(OH)D) levels and numbers of circulatory naive, central memory (Tcm), and effector memory (Tem) T lymphocytes in a large population of healthy children. Methods: Among 3189 children participating in a population-based prospective cohort, we measured 25(OH)D levels and performed detailed immunophenotyping of naive and memory T lymphocytes at a median age of 6.0 years (95% range 5.7-7.9). Detailed lymphocyte subsets were available in 986 children. Multivariable linear regression analyses were performed to determine the association between 25(OH)D and the maturation of T lymphocytes in children adjusted for cord blood 25(OH)D levels, herpes seropositivity, sociodemographic and lifestyle confounders. Furthermore, multivariable logistic regression analyses were performed to determine associations between 25(OH)D and childhood infections. Results: Higher 25(OH)D levels were associated with higher numbers of Tem lymphocytes. Every 10 nmol/L higher 25(OH)D was associated with 2.20% (95% CI 0.54-3.89; P=.009) higher CD4TemRA, 1.50% (95% CI 0.38-2.62; P=.008) higher CD4TemRO, and 1.82% (95% CI 0.11-3.56; P=.037) higher CD8TemRA cell numbers. Generally, stronger associations were observed among boys. 25(OH)D levels were not significantly associated with naive, Tcm cell numbers, herpes seropositivity, or URTIs. Conclusions: Our results suggest that vitamin D enhances cellular immunity in young children.

Original languageEnglish
Pages (from-to)579-587
Number of pages9
JournalPediatric Allergy and Immunology
Volume28
Issue number6
DOIs
Publication statusPublished - 1 Sep 2017

Keywords

  • adaptive immune system
  • calcitriol
  • child
  • T lymphocytes
  • vitamin D

Cite this

Looman, K. I. M., Jansen, M. A. E., Voortman, T., van den Heuvel, D., Jaddoe, V. W. V., Franco, O. H., ... Moll, H. A. (2017). The role of vitamin D on circulating memory T cells in children: The Generation R study. Pediatric Allergy and Immunology, 28(6), 579-587. https://doi.org/10.1111/pai.12754
Looman, Kirsten I.M. ; Jansen, Michelle A.E. ; Voortman, Trudy ; van den Heuvel, Diana ; Jaddoe, Vincent W.V. ; Franco, Oscar H. ; van Zelm, Menno C. ; Moll, Henriëtte A. / The role of vitamin D on circulating memory T cells in children : The Generation R study. In: Pediatric Allergy and Immunology. 2017 ; Vol. 28, No. 6. pp. 579-587.
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title = "The role of vitamin D on circulating memory T cells in children: The Generation R study",
abstract = "Background: Previous studies have demonstrated that vitamin D affects T-cell function and maturation via the vitamin D receptor. However, no studies in children have been performed on this topic. Because most of the T-cell memory is formed in the first 5 years of life, we aimed to determine the association between serum 25-hydroxyvitamin D (25(OH)D) levels and numbers of circulatory naive, central memory (Tcm), and effector memory (Tem) T lymphocytes in a large population of healthy children. Methods: Among 3189 children participating in a population-based prospective cohort, we measured 25(OH)D levels and performed detailed immunophenotyping of naive and memory T lymphocytes at a median age of 6.0 years (95{\%} range 5.7-7.9). Detailed lymphocyte subsets were available in 986 children. Multivariable linear regression analyses were performed to determine the association between 25(OH)D and the maturation of T lymphocytes in children adjusted for cord blood 25(OH)D levels, herpes seropositivity, sociodemographic and lifestyle confounders. Furthermore, multivariable logistic regression analyses were performed to determine associations between 25(OH)D and childhood infections. Results: Higher 25(OH)D levels were associated with higher numbers of Tem lymphocytes. Every 10 nmol/L higher 25(OH)D was associated with 2.20{\%} (95{\%} CI 0.54-3.89; P=.009) higher CD4TemRA, 1.50{\%} (95{\%} CI 0.38-2.62; P=.008) higher CD4TemRO, and 1.82{\%} (95{\%} CI 0.11-3.56; P=.037) higher CD8TemRA cell numbers. Generally, stronger associations were observed among boys. 25(OH)D levels were not significantly associated with naive, Tcm cell numbers, herpes seropositivity, or URTIs. Conclusions: Our results suggest that vitamin D enhances cellular immunity in young children.",
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Looman, KIM, Jansen, MAE, Voortman, T, van den Heuvel, D, Jaddoe, VWV, Franco, OH, van Zelm, MC & Moll, HA 2017, 'The role of vitamin D on circulating memory T cells in children: The Generation R study', Pediatric Allergy and Immunology, vol. 28, no. 6, pp. 579-587. https://doi.org/10.1111/pai.12754

The role of vitamin D on circulating memory T cells in children : The Generation R study. / Looman, Kirsten I.M.; Jansen, Michelle A.E.; Voortman, Trudy; van den Heuvel, Diana; Jaddoe, Vincent W.V.; Franco, Oscar H.; van Zelm, Menno C.; Moll, Henriëtte A.

In: Pediatric Allergy and Immunology, Vol. 28, No. 6, 01.09.2017, p. 579-587.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The role of vitamin D on circulating memory T cells in children

T2 - The Generation R study

AU - Looman, Kirsten I.M.

AU - Jansen, Michelle A.E.

AU - Voortman, Trudy

AU - van den Heuvel, Diana

AU - Jaddoe, Vincent W.V.

AU - Franco, Oscar H.

AU - van Zelm, Menno C.

AU - Moll, Henriëtte A.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Background: Previous studies have demonstrated that vitamin D affects T-cell function and maturation via the vitamin D receptor. However, no studies in children have been performed on this topic. Because most of the T-cell memory is formed in the first 5 years of life, we aimed to determine the association between serum 25-hydroxyvitamin D (25(OH)D) levels and numbers of circulatory naive, central memory (Tcm), and effector memory (Tem) T lymphocytes in a large population of healthy children. Methods: Among 3189 children participating in a population-based prospective cohort, we measured 25(OH)D levels and performed detailed immunophenotyping of naive and memory T lymphocytes at a median age of 6.0 years (95% range 5.7-7.9). Detailed lymphocyte subsets were available in 986 children. Multivariable linear regression analyses were performed to determine the association between 25(OH)D and the maturation of T lymphocytes in children adjusted for cord blood 25(OH)D levels, herpes seropositivity, sociodemographic and lifestyle confounders. Furthermore, multivariable logistic regression analyses were performed to determine associations between 25(OH)D and childhood infections. Results: Higher 25(OH)D levels were associated with higher numbers of Tem lymphocytes. Every 10 nmol/L higher 25(OH)D was associated with 2.20% (95% CI 0.54-3.89; P=.009) higher CD4TemRA, 1.50% (95% CI 0.38-2.62; P=.008) higher CD4TemRO, and 1.82% (95% CI 0.11-3.56; P=.037) higher CD8TemRA cell numbers. Generally, stronger associations were observed among boys. 25(OH)D levels were not significantly associated with naive, Tcm cell numbers, herpes seropositivity, or URTIs. Conclusions: Our results suggest that vitamin D enhances cellular immunity in young children.

AB - Background: Previous studies have demonstrated that vitamin D affects T-cell function and maturation via the vitamin D receptor. However, no studies in children have been performed on this topic. Because most of the T-cell memory is formed in the first 5 years of life, we aimed to determine the association between serum 25-hydroxyvitamin D (25(OH)D) levels and numbers of circulatory naive, central memory (Tcm), and effector memory (Tem) T lymphocytes in a large population of healthy children. Methods: Among 3189 children participating in a population-based prospective cohort, we measured 25(OH)D levels and performed detailed immunophenotyping of naive and memory T lymphocytes at a median age of 6.0 years (95% range 5.7-7.9). Detailed lymphocyte subsets were available in 986 children. Multivariable linear regression analyses were performed to determine the association between 25(OH)D and the maturation of T lymphocytes in children adjusted for cord blood 25(OH)D levels, herpes seropositivity, sociodemographic and lifestyle confounders. Furthermore, multivariable logistic regression analyses were performed to determine associations between 25(OH)D and childhood infections. Results: Higher 25(OH)D levels were associated with higher numbers of Tem lymphocytes. Every 10 nmol/L higher 25(OH)D was associated with 2.20% (95% CI 0.54-3.89; P=.009) higher CD4TemRA, 1.50% (95% CI 0.38-2.62; P=.008) higher CD4TemRO, and 1.82% (95% CI 0.11-3.56; P=.037) higher CD8TemRA cell numbers. Generally, stronger associations were observed among boys. 25(OH)D levels were not significantly associated with naive, Tcm cell numbers, herpes seropositivity, or URTIs. Conclusions: Our results suggest that vitamin D enhances cellular immunity in young children.

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KW - calcitriol

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Looman KIM, Jansen MAE, Voortman T, van den Heuvel D, Jaddoe VWV, Franco OH et al. The role of vitamin D on circulating memory T cells in children: The Generation R study. Pediatric Allergy and Immunology. 2017 Sep 1;28(6):579-587. https://doi.org/10.1111/pai.12754