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The role of secreted frizzled related protein 4 (sFRP-4) in regulating oestradiol-induced growth of the MCF-7 breast cancer cell line

  • Sally McLaren
  • , Frank Arfuso
  • , Nik Zeps
  • , Arun Dharmarajan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The Wnt signalling pathway is involved in regulating cellular proliferation and differentiation, and aberrant activation has been described in several cancers including breast. Oestradiol up regulates Wnt pathway gene expression, and thereby activates the Wnt signalling pathway. We used the oestrogen-responsive breast cancer cell line MCF-7 to examine the effects of secreted frizzled related protein 4 (sFRP-4) on oestradiol-induced growth, including gene expression of the Wnt signalling pathway genes Frizzled Receptor, Wnt-10b, and β-catenin. We demonstrate here that sFRP-4 inhibits oestradiol-induced cell growth in the MCF-7 cell line and also down regulates oestradiol-induced expression of selected Wnt signalling genes including β-catenin. We propose that sFRP-4 is a potent inhibitor of the Wnt signalling pathway and may negatively regulate oestradiol-mediated proliferation in human breast cancer cells.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalJournal of Analytical Oncology
Volume3
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Breast cancer
  • cellular proliferation
  • Growth inhibition
  • oestradiol
  • sFRP4
  • Wnt signalling
  • β-catenin

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