TY - JOUR
T1 - The Role of Peripheral Myelin Protein 2 in Remyelination
AU - Stettner, Mark
AU - Zenker, Jennifer
AU - Klingler, Fabian
AU - Szepanowski, Fabian
AU - Hartung, Hans P.
AU - Mausberg, Anne K.
AU - Kleinschnitz, Christoph
AU - Chrast, Roman
AU - Kieseier, Bernd C.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - The protein component of the myelin layer is essential for all aspects of peripheral nerves, and its deficiency can lead to structural and functional impairment. The presence of peripheral myelin protein 2 (P2, PMP2, FABP8, M-FABP) in Schwann cells has been known for decades and shown recently to be involved in the lipid homeostasis in the peripheral neural system. However, its precise role during de- and remyelination has yet to be elucidated. To this end, we assessed remyelination after sciatic nerve crush injury in vivo, and in an experimental de/remyelination ex vivo myelinating culture model in P2-deficient (P2−/−) and wild-type (WT) animals. In vivo, the nerve crush paradigm revealed temporal structural and functional changes in P2−/− mice as compared to WT animals. Concomitantly, P2−/− DRG cultures demonstrated the presence of shorter internodes and enlarged nodes after ex vivo de/remyelination. Together, these data indicate that P2 may play a role in remyelination of the injured peripheral nervous system, presumably by affecting the nodal and internodal configuration.
AB - The protein component of the myelin layer is essential for all aspects of peripheral nerves, and its deficiency can lead to structural and functional impairment. The presence of peripheral myelin protein 2 (P2, PMP2, FABP8, M-FABP) in Schwann cells has been known for decades and shown recently to be involved in the lipid homeostasis in the peripheral neural system. However, its precise role during de- and remyelination has yet to be elucidated. To this end, we assessed remyelination after sciatic nerve crush injury in vivo, and in an experimental de/remyelination ex vivo myelinating culture model in P2-deficient (P2−/−) and wild-type (WT) animals. In vivo, the nerve crush paradigm revealed temporal structural and functional changes in P2−/− mice as compared to WT animals. Concomitantly, P2−/− DRG cultures demonstrated the presence of shorter internodes and enlarged nodes after ex vivo de/remyelination. Together, these data indicate that P2 may play a role in remyelination of the injured peripheral nervous system, presumably by affecting the nodal and internodal configuration.
KW - Chronic inflammatory demyelinating neuropathy
KW - CIDP
KW - Dorsal root ganglia
KW - Nerve crush injury
KW - Neuropathy
KW - P2
KW - Peripheral myelin
UR - http://www.scopus.com/inward/record.url?scp=85018358290&partnerID=8YFLogxK
U2 - 10.1007/s10571-017-0494-0
DO - 10.1007/s10571-017-0494-0
M3 - Article
C2 - 28447247
AN - SCOPUS:85018358290
SN - 0272-4340
VL - 38
SP - 487
EP - 496
JO - Cellular and Molecular Neurobiology
JF - Cellular and Molecular Neurobiology
IS - 2
M1 - 28447247
ER -