The role of peptide loops of the Bordetella pertussis protein P.69 pertactin in antibody recognition

Marcel Hijnen, Richarda de Voer, Frits R Mooi, Rutger Schepp, Ed E Moret, Pieter van Gageldonk, Gaby Smits, Guy AM Berbers

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Bordetella pertussis, the etiological agent of whooping cough, is re-emerging in several countries with a traditionally high vaccine uptake. In these B. pertussis strains, polymorphisms were found in several proteins, including P.69 pertactin (P.69 Prn). P.69 Prn, an adhesin, contains two variable regions which are composed of repeats, one of which flanks the receptor binding site. Antibody titers against P.69 Prn correlate with protection and P.69 Prn is one of the components of acellular pertussis vaccines. Nevertheless, little is known about the structure and location of P.69 Prn epitopes. We used a three pronged approach to identify discontinuous epitopes that are recognized by mouse monoclonal antibodies, i.e. site-directed mutagenesis, deletion mapping and competition assays. Site-directed mutagenesis was focused on regions of P.69 Prn predicted to form loops according to the crystal structure. In this report we describe the location of several discontinuous epitopes that are also recognized by human antibodies. Our results reveal an important role of the N-terminus in immune recognition. We provide data for an indirect role of loops in immune evasion by masking of epitopes. We propose that the repeat regions have evolved to allow rapid antigenic variation to deflect the immune response from the functional domain of P.69 Prn. The results presented here provide a better understanding of the structure and function of variable loops and their role in the persistence of pathogens in immunologically primed populations.
Original languageEnglish
Pages (from-to)5902 - 5914
Number of pages13
JournalVaccine
Volume25
Issue number31
DOIs
Publication statusPublished - 2007

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