The role of lung inflation and sodium transport in airway liquid clearance during lung aeration in newborn rabbits

Melissa Li-Lian Siew, Megan Jane Wallace, Beth Joyce Allison, Marcus John Kitchen, Arjan B Te Pas, Muhammad Sirajul Islam, Robert A Lewis, Andreas Fouras, Naoto Yagi, Kentaro Uesugi, Stuart Brian Hooper

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background:Recent phase-contrast X-ray imaging studies suggest that inspiration primarily drives lung aeration and airway liquid clearance at birth, which questions the role of adrenaline-induced activation of epithelial sodium channels (ENaCs). We hypothesized that pressures generated by inspiration have a greater role in airway liquid clearance than do ENaCs after birth.Methods:Rabbit pups (30 d of gestation) were delivered and sedated, and 0.1 ml of saline (S) or amiloride (Am; an ENaC inhibitor) was instilled into the lungs before mechanical ventilation. Two other groups (30 d of gestation) were treated similarly but were also given adrenaline (S/Ad and Am/Ad) before mechanical ventilation.Results:Amiloride and adrenaline did not affect functional residual capacity (FRC) recruitment (P > 0.05). Amiloride increased the rate of FRC loss between inflations (Am: -5.2 ? 0.6 ml/kg/s), whereas adrenaline reduced the rate of FRC loss (S/Ad: -1.9 ? 0.3 ml/kg/s) as compared with saline-treated controls (S: -3.5 ? -0.6 ml/kg/s; P <0.05).Conclusion:These data indicate that inspiration is a major determinant of airway liquid clearance and FRC development during positive pressure ventilation. Although ENaC inhibition and adrenaline administration had no detectable effect on FRC development, ENaC may help to prevent liquid from re-entering the airways during expiration.
Original languageEnglish
Pages (from-to)443 - 449
Number of pages7
JournalPediatric Research
Volume73
Issue number4
DOIs
Publication statusPublished - 2013

Cite this

Siew, Melissa Li-Lian ; Wallace, Megan Jane ; Allison, Beth Joyce ; Kitchen, Marcus John ; Te Pas, Arjan B ; Islam, Muhammad Sirajul ; Lewis, Robert A ; Fouras, Andreas ; Yagi, Naoto ; Uesugi, Kentaro ; Hooper, Stuart Brian. / The role of lung inflation and sodium transport in airway liquid clearance during lung aeration in newborn rabbits. In: Pediatric Research. 2013 ; Vol. 73, No. 4. pp. 443 - 449.
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title = "The role of lung inflation and sodium transport in airway liquid clearance during lung aeration in newborn rabbits",
abstract = "Background:Recent phase-contrast X-ray imaging studies suggest that inspiration primarily drives lung aeration and airway liquid clearance at birth, which questions the role of adrenaline-induced activation of epithelial sodium channels (ENaCs). We hypothesized that pressures generated by inspiration have a greater role in airway liquid clearance than do ENaCs after birth.Methods:Rabbit pups (30 d of gestation) were delivered and sedated, and 0.1 ml of saline (S) or amiloride (Am; an ENaC inhibitor) was instilled into the lungs before mechanical ventilation. Two other groups (30 d of gestation) were treated similarly but were also given adrenaline (S/Ad and Am/Ad) before mechanical ventilation.Results:Amiloride and adrenaline did not affect functional residual capacity (FRC) recruitment (P > 0.05). Amiloride increased the rate of FRC loss between inflations (Am: -5.2 ? 0.6 ml/kg/s), whereas adrenaline reduced the rate of FRC loss (S/Ad: -1.9 ? 0.3 ml/kg/s) as compared with saline-treated controls (S: -3.5 ? -0.6 ml/kg/s; P <0.05).Conclusion:These data indicate that inspiration is a major determinant of airway liquid clearance and FRC development during positive pressure ventilation. Although ENaC inhibition and adrenaline administration had no detectable effect on FRC development, ENaC may help to prevent liquid from re-entering the airways during expiration.",
author = "Siew, {Melissa Li-Lian} and Wallace, {Megan Jane} and Allison, {Beth Joyce} and Kitchen, {Marcus John} and {Te Pas}, {Arjan B} and Islam, {Muhammad Sirajul} and Lewis, {Robert A} and Andreas Fouras and Naoto Yagi and Kentaro Uesugi and Hooper, {Stuart Brian}",
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The role of lung inflation and sodium transport in airway liquid clearance during lung aeration in newborn rabbits. / Siew, Melissa Li-Lian; Wallace, Megan Jane; Allison, Beth Joyce; Kitchen, Marcus John; Te Pas, Arjan B; Islam, Muhammad Sirajul; Lewis, Robert A; Fouras, Andreas; Yagi, Naoto; Uesugi, Kentaro; Hooper, Stuart Brian.

In: Pediatric Research, Vol. 73, No. 4, 2013, p. 443 - 449.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The role of lung inflation and sodium transport in airway liquid clearance during lung aeration in newborn rabbits

AU - Siew, Melissa Li-Lian

AU - Wallace, Megan Jane

AU - Allison, Beth Joyce

AU - Kitchen, Marcus John

AU - Te Pas, Arjan B

AU - Islam, Muhammad Sirajul

AU - Lewis, Robert A

AU - Fouras, Andreas

AU - Yagi, Naoto

AU - Uesugi, Kentaro

AU - Hooper, Stuart Brian

PY - 2013

Y1 - 2013

N2 - Background:Recent phase-contrast X-ray imaging studies suggest that inspiration primarily drives lung aeration and airway liquid clearance at birth, which questions the role of adrenaline-induced activation of epithelial sodium channels (ENaCs). We hypothesized that pressures generated by inspiration have a greater role in airway liquid clearance than do ENaCs after birth.Methods:Rabbit pups (30 d of gestation) were delivered and sedated, and 0.1 ml of saline (S) or amiloride (Am; an ENaC inhibitor) was instilled into the lungs before mechanical ventilation. Two other groups (30 d of gestation) were treated similarly but were also given adrenaline (S/Ad and Am/Ad) before mechanical ventilation.Results:Amiloride and adrenaline did not affect functional residual capacity (FRC) recruitment (P > 0.05). Amiloride increased the rate of FRC loss between inflations (Am: -5.2 ? 0.6 ml/kg/s), whereas adrenaline reduced the rate of FRC loss (S/Ad: -1.9 ? 0.3 ml/kg/s) as compared with saline-treated controls (S: -3.5 ? -0.6 ml/kg/s; P <0.05).Conclusion:These data indicate that inspiration is a major determinant of airway liquid clearance and FRC development during positive pressure ventilation. Although ENaC inhibition and adrenaline administration had no detectable effect on FRC development, ENaC may help to prevent liquid from re-entering the airways during expiration.

AB - Background:Recent phase-contrast X-ray imaging studies suggest that inspiration primarily drives lung aeration and airway liquid clearance at birth, which questions the role of adrenaline-induced activation of epithelial sodium channels (ENaCs). We hypothesized that pressures generated by inspiration have a greater role in airway liquid clearance than do ENaCs after birth.Methods:Rabbit pups (30 d of gestation) were delivered and sedated, and 0.1 ml of saline (S) or amiloride (Am; an ENaC inhibitor) was instilled into the lungs before mechanical ventilation. Two other groups (30 d of gestation) were treated similarly but were also given adrenaline (S/Ad and Am/Ad) before mechanical ventilation.Results:Amiloride and adrenaline did not affect functional residual capacity (FRC) recruitment (P > 0.05). Amiloride increased the rate of FRC loss between inflations (Am: -5.2 ? 0.6 ml/kg/s), whereas adrenaline reduced the rate of FRC loss (S/Ad: -1.9 ? 0.3 ml/kg/s) as compared with saline-treated controls (S: -3.5 ? -0.6 ml/kg/s; P <0.05).Conclusion:These data indicate that inspiration is a major determinant of airway liquid clearance and FRC development during positive pressure ventilation. Although ENaC inhibition and adrenaline administration had no detectable effect on FRC development, ENaC may help to prevent liquid from re-entering the airways during expiration.

UR - http://www.ncbi.nlm.nih.gov/pubmed/23269118

U2 - 10.1038/pr.2012.197

DO - 10.1038/pr.2012.197

M3 - Article

VL - 73

SP - 443

EP - 449

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 4

ER -