The role of follicular helper T cell molecules and environmental influences in autoantibody production and progression to inflammatory arthritis in mice

Nina Chevalier, Laurence Macia, Jian K. Tan, Linda J. Mason, Remy Robert, Alison N. Thorburn, Connie H. Y. Wong, Louis M. Tsai, Katherine Bourne, Robert Brink, Di Yu, Charles R. Mackay

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Objective: Antibody-mediated autoimmunity involves cognate interactions between self-reactive T cells and B cells during germinal center (GC) reactions. The aim of this study was to determine the role of essential follicular helper T (Tfh) cell molecules (CXCR5, signaling lymphocytic activation molecule–associated protein) on autoreactive CD4+ cells and the role of certain environmental influences that may determine GC-driven autoantibody production and arthritis development.
Methods: We transferred self-reactive CD4+ cells from KRN-Tg mice into recipient mice, which induced autoantibodies and autoinflammatory arthritis. This model allowed manipulation of environmental effects, such as inflammation, and use of transferred cells that were genetically deficient in important Tfh cell–associated molecules.
Results: A deficiency of signaling lymphocytic activation molecule–associated protein (SAP) in CD4+ cells from KRN-Tg mice completely protected against arthritis, indicating that stable T cell–B cell interactions are required for GC formation, autoantibody production, and arthritis induction. In contrast, a CXCR5 deficiency in CD4+ cells from KRN-Tg mice still induced disease when these cells were transferred into wild-type mice, suggesting that T cell help for B cells could rely on other migration mechanisms. However, various manipulations influenced this system, including elimination of bystander effects through use of CD28−/− recipient mice (reduced disease) or use of inflammation-inducing Freund's complete adjuvant (progression to arthritis). We also examined the capacity of preexisting GCs with a nonautoimmune specificity to co-opt autoimmune T cells and observed no evidence for any influence.
Conclusion: In addition to the quality and quantity of cognate CD4+ cell help, external factors such as inflammation and noncognate CD4+ cell bystander activation trigger autoimmunity by shaping events within autoimmune GC responses. SAP is an essential molecule for autoimmune antibody production, whereas the importance of CXCR5 varies depending on the circumstances.
Original languageEnglish
Pages (from-to)1026-1038
Number of pages13
JournalArthritis and Rheumatology
Volume68
Issue number4
DOIs
Publication statusPublished - 4 Apr 2016

Cite this

Chevalier, Nina ; Macia, Laurence ; Tan, Jian K. ; Mason, Linda J. ; Robert, Remy ; Thorburn, Alison N. ; Wong, Connie H. Y. ; Tsai, Louis M. ; Bourne, Katherine ; Brink, Robert ; Yu, Di ; Mackay, Charles R. / The role of follicular helper T cell molecules and environmental influences in autoantibody production and progression to inflammatory arthritis in mice. In: Arthritis and Rheumatology. 2016 ; Vol. 68, No. 4. pp. 1026-1038.
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title = "The role of follicular helper T cell molecules and environmental influences in autoantibody production and progression to inflammatory arthritis in mice",
abstract = "Objective: Antibody-mediated autoimmunity involves cognate interactions between self-reactive T cells and B cells during germinal center (GC) reactions. The aim of this study was to determine the role of essential follicular helper T (Tfh) cell molecules (CXCR5, signaling lymphocytic activation molecule–associated protein) on autoreactive CD4+ cells and the role of certain environmental influences that may determine GC-driven autoantibody production and arthritis development.Methods: We transferred self-reactive CD4+ cells from KRN-Tg mice into recipient mice, which induced autoantibodies and autoinflammatory arthritis. This model allowed manipulation of environmental effects, such as inflammation, and use of transferred cells that were genetically deficient in important Tfh cell–associated molecules.Results: A deficiency of signaling lymphocytic activation molecule–associated protein (SAP) in CD4+ cells from KRN-Tg mice completely protected against arthritis, indicating that stable T cell–B cell interactions are required for GC formation, autoantibody production, and arthritis induction. In contrast, a CXCR5 deficiency in CD4+ cells from KRN-Tg mice still induced disease when these cells were transferred into wild-type mice, suggesting that T cell help for B cells could rely on other migration mechanisms. However, various manipulations influenced this system, including elimination of bystander effects through use of CD28−/− recipient mice (reduced disease) or use of inflammation-inducing Freund's complete adjuvant (progression to arthritis). We also examined the capacity of preexisting GCs with a nonautoimmune specificity to co-opt autoimmune T cells and observed no evidence for any influence.Conclusion: In addition to the quality and quantity of cognate CD4+ cell help, external factors such as inflammation and noncognate CD4+ cell bystander activation trigger autoimmunity by shaping events within autoimmune GC responses. SAP is an essential molecule for autoimmune antibody production, whereas the importance of CXCR5 varies depending on the circumstances.",
author = "Nina Chevalier and Laurence Macia and Tan, {Jian K.} and Mason, {Linda J.} and Remy Robert and Thorburn, {Alison N.} and Wong, {Connie H. Y.} and Tsai, {Louis M.} and Katherine Bourne and Robert Brink and Di Yu and Mackay, {Charles R.}",
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The role of follicular helper T cell molecules and environmental influences in autoantibody production and progression to inflammatory arthritis in mice. / Chevalier, Nina; Macia, Laurence; Tan, Jian K.; Mason, Linda J.; Robert, Remy; Thorburn, Alison N.; Wong, Connie H. Y.; Tsai, Louis M.; Bourne, Katherine; Brink, Robert; Yu, Di; Mackay, Charles R.

In: Arthritis and Rheumatology, Vol. 68, No. 4, 04.04.2016, p. 1026-1038.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The role of follicular helper T cell molecules and environmental influences in autoantibody production and progression to inflammatory arthritis in mice

AU - Chevalier, Nina

AU - Macia, Laurence

AU - Tan, Jian K.

AU - Mason, Linda J.

AU - Robert, Remy

AU - Thorburn, Alison N.

AU - Wong, Connie H. Y.

AU - Tsai, Louis M.

AU - Bourne, Katherine

AU - Brink, Robert

AU - Yu, Di

AU - Mackay, Charles R.

PY - 2016/4/4

Y1 - 2016/4/4

N2 - Objective: Antibody-mediated autoimmunity involves cognate interactions between self-reactive T cells and B cells during germinal center (GC) reactions. The aim of this study was to determine the role of essential follicular helper T (Tfh) cell molecules (CXCR5, signaling lymphocytic activation molecule–associated protein) on autoreactive CD4+ cells and the role of certain environmental influences that may determine GC-driven autoantibody production and arthritis development.Methods: We transferred self-reactive CD4+ cells from KRN-Tg mice into recipient mice, which induced autoantibodies and autoinflammatory arthritis. This model allowed manipulation of environmental effects, such as inflammation, and use of transferred cells that were genetically deficient in important Tfh cell–associated molecules.Results: A deficiency of signaling lymphocytic activation molecule–associated protein (SAP) in CD4+ cells from KRN-Tg mice completely protected against arthritis, indicating that stable T cell–B cell interactions are required for GC formation, autoantibody production, and arthritis induction. In contrast, a CXCR5 deficiency in CD4+ cells from KRN-Tg mice still induced disease when these cells were transferred into wild-type mice, suggesting that T cell help for B cells could rely on other migration mechanisms. However, various manipulations influenced this system, including elimination of bystander effects through use of CD28−/− recipient mice (reduced disease) or use of inflammation-inducing Freund's complete adjuvant (progression to arthritis). We also examined the capacity of preexisting GCs with a nonautoimmune specificity to co-opt autoimmune T cells and observed no evidence for any influence.Conclusion: In addition to the quality and quantity of cognate CD4+ cell help, external factors such as inflammation and noncognate CD4+ cell bystander activation trigger autoimmunity by shaping events within autoimmune GC responses. SAP is an essential molecule for autoimmune antibody production, whereas the importance of CXCR5 varies depending on the circumstances.

AB - Objective: Antibody-mediated autoimmunity involves cognate interactions between self-reactive T cells and B cells during germinal center (GC) reactions. The aim of this study was to determine the role of essential follicular helper T (Tfh) cell molecules (CXCR5, signaling lymphocytic activation molecule–associated protein) on autoreactive CD4+ cells and the role of certain environmental influences that may determine GC-driven autoantibody production and arthritis development.Methods: We transferred self-reactive CD4+ cells from KRN-Tg mice into recipient mice, which induced autoantibodies and autoinflammatory arthritis. This model allowed manipulation of environmental effects, such as inflammation, and use of transferred cells that were genetically deficient in important Tfh cell–associated molecules.Results: A deficiency of signaling lymphocytic activation molecule–associated protein (SAP) in CD4+ cells from KRN-Tg mice completely protected against arthritis, indicating that stable T cell–B cell interactions are required for GC formation, autoantibody production, and arthritis induction. In contrast, a CXCR5 deficiency in CD4+ cells from KRN-Tg mice still induced disease when these cells were transferred into wild-type mice, suggesting that T cell help for B cells could rely on other migration mechanisms. However, various manipulations influenced this system, including elimination of bystander effects through use of CD28−/− recipient mice (reduced disease) or use of inflammation-inducing Freund's complete adjuvant (progression to arthritis). We also examined the capacity of preexisting GCs with a nonautoimmune specificity to co-opt autoimmune T cells and observed no evidence for any influence.Conclusion: In addition to the quality and quantity of cognate CD4+ cell help, external factors such as inflammation and noncognate CD4+ cell bystander activation trigger autoimmunity by shaping events within autoimmune GC responses. SAP is an essential molecule for autoimmune antibody production, whereas the importance of CXCR5 varies depending on the circumstances.

UR - http://www.ncbi.nlm.nih.gov/pubmed/26501485

U2 - 10.1002/art.39481

DO - 10.1002/art.39481

M3 - Article

VL - 68

SP - 1026

EP - 1038

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 4

ER -