TY - JOUR
T1 - The respective roles of the protein kinase and pppA2′ p5′ A2′ p5′ a-activated endonuclease in the inhibition of protein synthesis by double stranded RNA in rabbit reticulocyte lysates
AU - Williams, Bryan R.G.
AU - Gilbert, Christopher S.
AU - Kerr, Ian M.
PY - 1979/4/1
Y1 - 1979/4/1
N2 - Double-stranded RNA (dsRNA) inhibits protein synthesis in rabbit reticulocyte lysates by activating the synthesis of the endonuclease effector pppA2′ p5′ A2′ p5′A (2-5A) and a protein kinase which phosphorylates the protein synthesis initiation factor eIF-2. Under certain assay conditions, high concentrations of dsRNA are without inhibitory effect in many lysates (high dsRNA "reversible" lysates). In these lysates natural dsRNA at low concentrations stimulated protein kinase activity to a greater extent than did the synthetic dsRNA poly rI.rC. Synthesis of 2-5A was greater when poly rI.rC was used. However, a number of factors, including the salt concentration and messenger RNA used, combine to determine the overall effect of dsRNA on protein synthesis under any given set of experimental conditions.
AB - Double-stranded RNA (dsRNA) inhibits protein synthesis in rabbit reticulocyte lysates by activating the synthesis of the endonuclease effector pppA2′ p5′ A2′ p5′A (2-5A) and a protein kinase which phosphorylates the protein synthesis initiation factor eIF-2. Under certain assay conditions, high concentrations of dsRNA are without inhibitory effect in many lysates (high dsRNA "reversible" lysates). In these lysates natural dsRNA at low concentrations stimulated protein kinase activity to a greater extent than did the synthetic dsRNA poly rI.rC. Synthesis of 2-5A was greater when poly rI.rC was used. However, a number of factors, including the salt concentration and messenger RNA used, combine to determine the overall effect of dsRNA on protein synthesis under any given set of experimental conditions.
UR - http://www.scopus.com/inward/record.url?scp=0018460608&partnerID=8YFLogxK
U2 - 10.1093/nar/6.4.1335
DO - 10.1093/nar/6.4.1335
M3 - Article
C2 - 450698
AN - SCOPUS:0018460608
SN - 0305-1048
VL - 6
SP - 1335
EP - 1350
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 4
ER -