The release of hepatitis B core antigen from Escherichia coli by batch mode bead milling

Chin Woi Ho, Wen Siang Tan, Suryani Kamarudin, Tau Chuan Ling, Beng Ti Tey

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The performance of a batch model bead mill on the release of hepatitis B core antigen (HBcAg) from Escherichia coli was investigated in this study. The operating parameters examined were impeller tip speed (8-14 m/s), biomass concentration [5-20% (w/v)] and bead loading [65-80% (v/v)]. The highest yield (24.3 mg/g cell) and rate constant (0.471 l/min) of HBcAg release were achieved at impeller tip speed of 14 m/s. However, the high-shear stress under these operating conditions caused damage of the HBcAg. The highest yield (22.7 mg/g cell) and rate constant (0.344 l/min) of HBcAg release were observed at biomass concentration of 20% (w/v). There was no significant effect of bead loading on the performance of bead milling being observed. In conclusion, the optimal operating condition for the release of HBcAg was at bead loading of 75% (v/v), biomass concentration of 20% (w/v) and impeller tip speed of 10 m/s.

Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalProcess Biochemistry
Volume43
Issue number2
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

Keywords

  • Batch mode
  • Bead mill
  • Cell disruption
  • Downstream process
  • Escherichia coli
  • Hepatitis B core antigen

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