The protein import channel in the outer mitosomal membrane of Giardia intestinalis

Michael J Dagley, Pavel Dolezal, Vladimir A Likic, Ondrej Smid, Anthony Wayne Purcell, Susan K Buchanan, Jan Tachezy, Trevor James Lithgow

Research output: Contribution to journalArticleResearchpeer-review

42 Citations (Scopus)

Abstract

The identification of mitosomes in Giardia generated significant debate on the evolutionary origin of these organelles; whether they were highly reduced mitochondria, or the product of a unique endosymbiotic event in an amitochondrial organism. As the protein import pathway is a defining characteristic of mitochondria, we sought to discover a TOM (translocase in the outer mitochondrial membrane) complex in Giardia. A Hidden Markov model search of the Giardia genome identified a Tom40 homologous sequence (GiTom40), where Tom40 is the protein translocation channel of the TOM complex. The GiTom40 protein is located in the membrane of mitosomes in a approximately 200 kDa TOM complex. As Tom40 was derived in the development of mitochondria to serve as the protein import channel in the outer membrane, its presence in Giardia evidences the mitochondrial ancestry of mitosomes.
Original languageEnglish
Pages (from-to)1941 - 1947
Number of pages7
JournalMolecular Biology and Evolution
Volume26
Issue number9
DOIs
Publication statusPublished - 2009

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