The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

Shen Y. Heazlewood; Brenda Williams; Melonie J Storan; Susan K. Nilsson

doi:10.1007/978-1-62703-508-8_10

The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

Shen Y. Heazlewood, Brenda Williams, Melonie J Storan, Susan K. Nilsson

Anatomy & Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

10 Citations (Scopus)

Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been diffi cult to isolate in large numbers. Here in, we report a fl uorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

Original language	English
Title of host publication	Stem Cell Niche: Methods and Protocols
Editors	Kursad Turksen
Publisher	Humana Press
Chapter	10
Pages	121-133
Number of pages	13
Volume	1035
ISBN (Print)	9781627035071
DOIs	https://doi.org/10.1007/978-1-62703-508-8_10
Publication status	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	1035
ISSN (Print)	10643745

Keywords

Bone marrow
Megakaryocytes
Microenvironment
Niche
Ploidy

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10.1007/978-1-62703-508-8_10

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Heazlewood, S. Y., Williams, B., Storan, M. J., & Nilsson, S. K. (2013). The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting. In K. Turksen (Ed.), Stem Cell Niche: Methods and Protocols (Vol. 1035, pp. 121-133). (Methods in Molecular Biology; Vol. 1035). Humana Press. https://doi.org/10.1007/978-1-62703-508-8_10

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abstract = "Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been diffi cult to isolate in large numbers. Here in, we report a fl uorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).",

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author = "Heazlewood, {Shen Y.} and Brenda Williams and Storan, {Melonie J} and Nilsson, {Susan K.}",

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Heazlewood, SY, Williams, B, Storan, MJ & Nilsson, SK 2013, The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting. in K Turksen (ed.), Stem Cell Niche: Methods and Protocols. vol. 1035, Methods in Molecular Biology, vol. 1035, Humana Press, pp. 121-133. https://doi.org/10.1007/978-1-62703-508-8_10

The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting. / Heazlewood, Shen Y.; Williams, Brenda; Storan, Melonie J et al.

Stem Cell Niche: Methods and Protocols. ed. / Kursad Turksen. Vol. 1035 Humana Press, 2013. p. 121-133 (Methods in Molecular Biology; Vol. 1035).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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T1 - The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

AU - Heazlewood, Shen Y.

AU - Williams, Brenda

AU - Storan, Melonie J

AU - Nilsson, Susan K.

PY - 2013

Y1 - 2013

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AB - Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been diffi cult to isolate in large numbers. Here in, we report a fl uorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

KW - Bone marrow

KW - Megakaryocytes

KW - Microenvironment

KW - Niche

KW - Ploidy

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BT - Stem Cell Niche: Methods and Protocols

A2 - Turksen, Kursad

PB - Humana Press

ER -

The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

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