The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting

Shen Y. Heazlewood, Brenda Williams, Melonie J Storan, Susan K. Nilsson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

6 Citations (Scopus)

Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been diffi cult to isolate in large numbers. Here in, we report a fl uorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

Original languageEnglish
Title of host publicationStem Cell Niche: Methods and Protocols
EditorsKursad Turksen
PublisherHumana Press
Chapter10
Pages121-133
Number of pages13
Volume1035
ISBN (Print)9781627035071
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)10643745

Keywords

  • Bone marrow
  • Megakaryocytes
  • Microenvironment
  • Niche
  • Ploidy

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