Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been diffi cult to isolate in large numbers. Here in, we report a fl uorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).
|Title of host publication||Stem Cell Niche: Methods and Protocols|
|Number of pages||13|
|Publication status||Published - 2013|
|Name||Methods in Molecular Biology|
- Bone marrow