The production of pluripotent stem cells from mouse Amniotic Fluid cells using a transposon system

Enrica Bertin, Martina Piccoli, Chiara Franzin, Andras Nagy, Maria Mileikovsky, Paolo De Coppi, Michela Pozzobon

Research output: Contribution to journalArticleOtherpeer-review

Abstract

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.

Original languageEnglish
Article numbere54598
JournalJournal of Visualized Experiments
Volume2017
Issue number120
DOIs
Publication statusPublished - 28 Feb 2017
Externally publishedYes

Keywords

  • Amniotic fluid cells
  • Developmental Biology
  • Induced pluripotent stem cells
  • Issue 120
  • Mouse
  • PiggyBac transposon
  • Regenerative medicine
  • Reprogramming

Cite this

Bertin, E., Piccoli, M., Franzin, C., Nagy, A., Mileikovsky, M., De Coppi, P., & Pozzobon, M. (2017). The production of pluripotent stem cells from mouse Amniotic Fluid cells using a transposon system. Journal of Visualized Experiments, 2017(120), [e54598]. https://doi.org/10.3791/54598
Bertin, Enrica ; Piccoli, Martina ; Franzin, Chiara ; Nagy, Andras ; Mileikovsky, Maria ; De Coppi, Paolo ; Pozzobon, Michela. / The production of pluripotent stem cells from mouse Amniotic Fluid cells using a transposon system. In: Journal of Visualized Experiments. 2017 ; Vol. 2017, No. 120.
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Bertin, E, Piccoli, M, Franzin, C, Nagy, A, Mileikovsky, M, De Coppi, P & Pozzobon, M 2017, 'The production of pluripotent stem cells from mouse Amniotic Fluid cells using a transposon system' Journal of Visualized Experiments, vol. 2017, no. 120, e54598. https://doi.org/10.3791/54598

The production of pluripotent stem cells from mouse Amniotic Fluid cells using a transposon system. / Bertin, Enrica; Piccoli, Martina; Franzin, Chiara; Nagy, Andras; Mileikovsky, Maria; De Coppi, Paolo; Pozzobon, Michela.

In: Journal of Visualized Experiments, Vol. 2017, No. 120, e54598, 28.02.2017.

Research output: Contribution to journalArticleOtherpeer-review

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