@article{e1c9d2ae1aa142f5ab7560bbeff28663,
title = "The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients",
abstract = "Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-na{\"i}ve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower transcriptional activity despite a high presence. The Bifidobacteriaceae family of the Actinobacteria phylum showed lower abundance in the active microbiota, although no difference in presence was detected. The most abundant microbiota members of the inflamed tissue in UC patients were not the most active. Knowledge of active members of microbiota in UC patients could enhance our understanding of disease etiology. The active microbial community composition did not deviate from the total when comparing UC patients to non-IBD controls.",
author = "Moen, {Aina E.Fossum} and Lindstr{\o}m, {Jonas Christoffer} and Tann{\ae}s, {Tone M{\o}ller} and Simen Vatn and Petr Ricanek and Vatn, {Morten H.} and J{\o}rgen Jahnsen and {The IBD-Character Consortium} and Frengen, {Anna B.} and Dahl, {Fredrik A.} and Panpan You and Janne S{\o}lvernes and Ekeland, {Gunn S.} and Detlie, {Trond E.} and Christine Olbj{\o}rn and O{\textquoteright}Leary, {Kate R.} and Ventham, {Nicholas T.} and Kennedy, {Nicholas A.} and Rahul Kalla and Alex Adams and Drummond, {Hazel E.} and Ray Boyapati and Nimmo, {Elaine R.} and Wilson, {David C.} and Jack Satsangi and Heath, {Simon C.} and Marta Gut and Angelika Merkel and Monica Bayes and Gut, {Ivo G.} and Keita, {{\AA}sa V.} and S{\"o}derholm, {Johan D.} and Henrik Hjortswang and Adam Carstens and Daniel Bergemalm and Jonas Halfvarson and Erik Andersson and M{\aa}rten Lindqvist and Dirk Repsilber and Marieke Pierik and Daisy Jonkers and Fernando Gomoll{\'o}n and Mauro D{\textquoteright}Amato and Leif T{\"o}rkvist and Fredrik Hjelm and Mats Gullberg and Niklas Nordberg and Anette Ocklind and Erik Pettersson and Daniel Ekman and Mikael Sundell and Eddie Modig and Ferdinando Bonfiglio and Veillard, {Anne Cl{\'e}mence} and Renaud Schoemans and Dominique Poncelet and C{\'e}line Sabatel and Torbj{\o}rn Lindahl and Ewa Ciemniejewska and Christina Cas{\'e}n and Charles Lees and Noble, {Colin L.} and Ian Arnott and Ho, {Gwo Tzer} and Shand, {Alan G.}",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41598-018-35243-4",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}