TY - JOUR
T1 - The potentiation of beta-lactam and anti-bacterial activities of lipophilic constituents from Mesua ferrae leaves against methicillin-resistant Staphylococcus aureus
AU - Alagasamy, Sangeeta V.
AU - Ramanathan, Surash
AU - Chear, Nelson Jeng Yeou
AU - Tan, Wen Nee
AU - Ramachandram, Dinesh Sangarran
AU - Ching-Ga, Ai Fein T.
AU - Ponnusamy, Yasodha
AU - Lai, Choon Sheen
AU - Murugaiyah, Vikneswaran
N1 - Funding Information:
Research funding: This study was supported by Universiti Sains Malaysia under the Research University Grant (1001/CDADAH/815096). Nelson Jeng-Yeou Chear and Dinesh Sangarran Ramachandram are the recipients of USM Fellowship Scheme funded by the Institute of Postgraduate Studies, Universiti Sains Malaysia. Ai-Fein T. Ching-Ga is the recipient of USM Graduate-Assistant Scheme funded by the Institute of Postgraduate Studies, Universiti Sains Malaysia.
Publisher Copyright:
© 2020 2020 Walter de Gruyter GmbH, Berlin/Boston.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/6/1
Y1 - 2021/6/1
N2 - Mesua ferrae, from the family of Calophyllaceae, is traditionally used for the treatment of piles, fever and renal disorders. The present study was aimed to examine the antibacterial compounds from the leaves of M. ferrae and their β-lactam antibiotic potentiate activities against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). Stigmasterol (1) and β-caryophyllene oxide (2) were isolated from the n-hexane fraction of the leaves of M. ferrae using a bioassay-guided fractionation approach. The isolated compounds displayed anti-Staphylococcus and anti-MRSA activities. It is worth to note that both compounds demonstrated synergism with β-lactam antibiotics against S. aureus and MRSA. Gas chromatography-mass spectrometry (GC-MS) analysis indicated the n-hexane fraction was dominated by triterpenes and sesquiterpenes, suggesting the total antibacterial activity exhibited by the fraction. Based on the findings, it could conclude that M. ferrae is a promising natural source for the discovery of new anti-MRSA lead compounds.
AB - Mesua ferrae, from the family of Calophyllaceae, is traditionally used for the treatment of piles, fever and renal disorders. The present study was aimed to examine the antibacterial compounds from the leaves of M. ferrae and their β-lactam antibiotic potentiate activities against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA). Stigmasterol (1) and β-caryophyllene oxide (2) were isolated from the n-hexane fraction of the leaves of M. ferrae using a bioassay-guided fractionation approach. The isolated compounds displayed anti-Staphylococcus and anti-MRSA activities. It is worth to note that both compounds demonstrated synergism with β-lactam antibiotics against S. aureus and MRSA. Gas chromatography-mass spectrometry (GC-MS) analysis indicated the n-hexane fraction was dominated by triterpenes and sesquiterpenes, suggesting the total antibacterial activity exhibited by the fraction. Based on the findings, it could conclude that M. ferrae is a promising natural source for the discovery of new anti-MRSA lead compounds.
KW - antibacterial
KW - Mesua ferrae
KW - MRSA
KW - n-hexane fraction
KW - Staphylococcus aureus
KW - stigmasterol
KW - synergism
KW - β-caryophyllene oxide
UR - http://www.scopus.com/inward/record.url?scp=85098883296&partnerID=8YFLogxK
U2 - 10.1515/jcim-2019-0316
DO - 10.1515/jcim-2019-0316
M3 - Article
C2 - 34187118
AN - SCOPUS:85098883296
SN - 1553-3840
VL - 18
SP - 339
EP - 345
JO - Journal of Complementary and Integrative Medicine
JF - Journal of Complementary and Integrative Medicine
IS - 2
ER -