The piggyBac transposon as a platform technology for somatic cell reprogramming studies in mouse

Knut Woltjen, Shin Il Kim, Andras Nagy

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

6 Citations (Scopus)

Abstract

Somatic cell reprogramming to induced pluripotent stem cells (iPSCs) is a revolutionary technology, with repercussions affecting modern functional genomics and regenerative medicine. Still, relatively little is known about the processes underlying this dramatic cellular and molecular metamorphosis. Reprogramming technology based on the implementation of piggyBac (PB) transposons has enabled studies of iPSC reprogramming mechanisms, shedding an increasing light on these processes. Unique characteristics of PB transposons such as efficient genomic integration, unlimited cargo capacity, robust gene expression, and even seamless excision highlight the importance of this transgenic tool in advancing stem cell biology. In this chapter, we provide a detailed overview of versatile primary iPSC generation from mouse somatic cells using PB transposons, and the subsequent establishment of robust secondary reprogramming systems. These protocols are highlighted with examples from recent studies as to how PB has been, and continues to be, conducive to the dissection of reprogramming processes at the cellular and molecular levels.

Original languageEnglish
Title of host publicationInduced Pluripotent Stem (iPS) Cells
EditorsKursad Turksen, Andras Nagy
Place of PublicationNew York, NY
PublisherHumana Press
Number of pages22
Volume1357
ISBN (Electronic)9781493930555
ISBN (Print)9781493930548
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)1064-3745

Keywords

  • Doxycycline regulation
  • Induced pluripotent stem cells (iPSCs)
  • PiggyBac
  • Reprogramming
  • Secondary reprogramming
  • Transposon

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