The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes

Yogitha N. Srikhanta; Tina L. Maguire; Katryn J. Stacey; Sean M. Grimmond; Michael P. Jennings

doi:10.1073/pnas.0501169102

The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes

Yogitha N. Srikhanta
, Tina L. Maguire
, Katryn J. Stacey
, Sean M. Grimmond
, Michael P. Jennings

Research output: Contribution to journal › Article › Research › peer-review

168 Citations (Scopus)

Abstract

Several host-adapted bacterial pathogens contain methyltransferases associated with type III restriction-modification (R-M) systems that are subject to reversible, high-frequency on/off switching of expression (phase variation). To investigate the role of phase-variable expression of R-M systems, we made a mutant strain lacking the methyltransferase (mod) associated with a type III R-M system of Haemophilus influenzas and analyzed its phenotype. By microarray analysis, we identified a number of genes that were either up- or down-regulated in the mod mutant strain. This system reports the coordinated random switching of a set of genes in a bacterial pathogen and may represent a widely used mechanism.

Original language	English
Pages (from-to)	5547-5551
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	15
DOIs	https://doi.org/10.1073/pnas.0501169102
Publication status	Published - 12 Apr 2005
Externally published	Yes

Keywords

DNA methyltransferase
Haemophilus influenzae
Phase variation

Access to Document

10.1073/pnas.0501169102

Cite this

@article{cc0464d9eabf431a954c4f0e535e0b77,

title = "The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes",

abstract = "Several host-adapted bacterial pathogens contain methyltransferases associated with type III restriction-modification (R-M) systems that are subject to reversible, high-frequency on/off switching of expression (phase variation). To investigate the role of phase-variable expression of R-M systems, we made a mutant strain lacking the methyltransferase (mod) associated with a type III R-M system of Haemophilus influenzas and analyzed its phenotype. By microarray analysis, we identified a number of genes that were either up- or down-regulated in the mod mutant strain. This system reports the coordinated random switching of a set of genes in a bacterial pathogen and may represent a widely used mechanism.",

keywords = "DNA methyltransferase, Haemophilus influenzae, Phase variation",

author = "Srikhanta, \{Yogitha N.\} and Maguire, \{Tina L.\} and Stacey, \{Katryn J.\} and Grimmond, \{Sean M.\} and Jennings, \{Michael P.\}",

year = "2005",

month = apr,

day = "12",

doi = "10.1073/pnas.0501169102",

language = "English",

volume = "102",

pages = "5547--5551",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "15",

}

The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes. / Srikhanta, Yogitha N.; Maguire, Tina L.; Stacey, Katryn J. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 15, 12.04.2005, p. 5547-5551.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - The phasevarion

T2 - A genetic system controlling coordinated, random switching of expression of multiple genes

AU - Srikhanta, Yogitha N.

AU - Maguire, Tina L.

AU - Stacey, Katryn J.

AU - Grimmond, Sean M.

AU - Jennings, Michael P.

PY - 2005/4/12

Y1 - 2005/4/12

N2 - Several host-adapted bacterial pathogens contain methyltransferases associated with type III restriction-modification (R-M) systems that are subject to reversible, high-frequency on/off switching of expression (phase variation). To investigate the role of phase-variable expression of R-M systems, we made a mutant strain lacking the methyltransferase (mod) associated with a type III R-M system of Haemophilus influenzas and analyzed its phenotype. By microarray analysis, we identified a number of genes that were either up- or down-regulated in the mod mutant strain. This system reports the coordinated random switching of a set of genes in a bacterial pathogen and may represent a widely used mechanism.

AB - Several host-adapted bacterial pathogens contain methyltransferases associated with type III restriction-modification (R-M) systems that are subject to reversible, high-frequency on/off switching of expression (phase variation). To investigate the role of phase-variable expression of R-M systems, we made a mutant strain lacking the methyltransferase (mod) associated with a type III R-M system of Haemophilus influenzas and analyzed its phenotype. By microarray analysis, we identified a number of genes that were either up- or down-regulated in the mod mutant strain. This system reports the coordinated random switching of a set of genes in a bacterial pathogen and may represent a widely used mechanism.

KW - DNA methyltransferase

KW - Haemophilus influenzae

KW - Phase variation

UR - https://www.scopus.com/pages/publications/17344370012

U2 - 10.1073/pnas.0501169102

DO - 10.1073/pnas.0501169102

M3 - Article

C2 - 15802471

AN - SCOPUS:17344370012

SN - 0027-8424

VL - 102

SP - 5547

EP - 5551

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 15

ER -

The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes

Abstract

Keywords

Access to Document

Other files and links

Cite this