The pH low insertion peptide pHLIP variant 3 as a novel marker of acidic malignant lesions

Thomas T. Tapmeier, Anna Moshnikova, John Beech, Danny Allen, Paul Kinchesh, Sean Smart, Adrian Harris, Alan McIntyre, Donald M. Engelman, Oleg A. Andreev, Yana K. Reshetnyak, Ruth J. Muschel

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37 Citations (Scopus)

Abstract

Current strategies for early detection of breast and other cancers are limited in part because some lesions identified as potentially malignant do not develop into aggressive tumors. Acid pH has been suggested as a key characteristic of aggressive tumors that might distinguish aggressive lesions from more indolent pathology. We therefore investigated the novel class of molecules, pH low insertion peptides (pHLIPs), as markers of low pH in tumor allografts and of malignant lesions in a mouse model of spontaneous breast cancer, BALB/neu-T. pHLIP Variant 3 (Var3) conjugated with fluorescent Alexa546 was shown to insert into tumor spheroids in a sequence-specific manner. Its signal reflected pH in murine tumors. It was induced by carbonic anhydrase IX (CAIX) overexpression and inhibited by acetazolamide (AZA) administration. By using 31P magnetic resonance spectroscopy (MRS), we demonstrated that pHLIP Var3 was retained in tumors of pH equal to or less than 6.7 but not in tissues of higher pH. In BALB/neu-T mice at different stages of the disease, the fluorescent signal from pHLIP Var3 marked cancerous lesions with a very low false-positive rate. However, only ∼60% of the smallest lesions retained a pHLIP Var3 signal, suggesting heterogeneity in pH. Taken together, these results show that pHLIP can identify regions of lower pH, allowing for its development as a theranostic tool for clinical applications.

Original languageEnglish
Pages (from-to)9710-9715
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number31
DOIs
Publication statusPublished - 4 Aug 2015
Externally publishedYes

Keywords

  • Carbonic anhydrase IX
  • Early detection
  • In vivo fluorescence
  • Invasive tumor
  • PHLIP

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