The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis

Abu Iftiaf Md Salah Ud-Din, Anna Roujeinikova

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Flagella-mediated motility and chemotaxis towards nutrients are important characteristics of Vibrio fischeri that play a crucial role in the development of its symbiotic relationship with its Hawaiian squid host Euprymna scolopes. The V. fischeri chemoreceptor A (VfcA) mediates chemotaxis toward amino acids. The periplasmic sensory domain of VfcA has been crystallized by the hangingdrop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. The crystals belonged to space group P1, with unit-cell parameters a = 39.9, b = 57.0, c = 117.0 A, a = 88.9, B = 80.5, y = 89.7 . A complete X-ray diffraction data set has been collected to 1.8 A ? resolution using cryocooling conditions and synchrotron radiation.
Original languageEnglish
Pages (from-to)382-385
Number of pages4
JournalActa Crystallographica. Section F: Structural Biology Communications
VolumeF72
Issue numberPart 5
DOIs
Publication statusPublished - 1 May 2016

Keywords

  • bacterial chemotaxis
  • methyl-accepting protein
  • receptor
  • sensing domain

Cite this

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title = "The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis",
abstract = "Flagella-mediated motility and chemotaxis towards nutrients are important characteristics of Vibrio fischeri that play a crucial role in the development of its symbiotic relationship with its Hawaiian squid host Euprymna scolopes. The V. fischeri chemoreceptor A (VfcA) mediates chemotaxis toward amino acids. The periplasmic sensory domain of VfcA has been crystallized by the hangingdrop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. The crystals belonged to space group P1, with unit-cell parameters a = 39.9, b = 57.0, c = 117.0 A, a = 88.9, B = 80.5, y = 89.7 . A complete X-ray diffraction data set has been collected to 1.8 A ? resolution using cryocooling conditions and synchrotron radiation.",
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author = "{Salah Ud-Din}, {Abu Iftiaf Md} and Anna Roujeinikova",
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The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis. / Salah Ud-Din, Abu Iftiaf Md; Roujeinikova, Anna.

In: Acta Crystallographica. Section F: Structural Biology Communications, Vol. F72, No. Part 5, 01.05.2016, p. 382-385.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The periplasmic sensing domain of Vibrio fischeri chemoreceptor protein A (VfcA): cloning, purification and crystallographic analysis

AU - Salah Ud-Din, Abu Iftiaf Md

AU - Roujeinikova, Anna

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Flagella-mediated motility and chemotaxis towards nutrients are important characteristics of Vibrio fischeri that play a crucial role in the development of its symbiotic relationship with its Hawaiian squid host Euprymna scolopes. The V. fischeri chemoreceptor A (VfcA) mediates chemotaxis toward amino acids. The periplasmic sensory domain of VfcA has been crystallized by the hangingdrop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. The crystals belonged to space group P1, with unit-cell parameters a = 39.9, b = 57.0, c = 117.0 A, a = 88.9, B = 80.5, y = 89.7 . A complete X-ray diffraction data set has been collected to 1.8 A ? resolution using cryocooling conditions and synchrotron radiation.

AB - Flagella-mediated motility and chemotaxis towards nutrients are important characteristics of Vibrio fischeri that play a crucial role in the development of its symbiotic relationship with its Hawaiian squid host Euprymna scolopes. The V. fischeri chemoreceptor A (VfcA) mediates chemotaxis toward amino acids. The periplasmic sensory domain of VfcA has been crystallized by the hangingdrop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. The crystals belonged to space group P1, with unit-cell parameters a = 39.9, b = 57.0, c = 117.0 A, a = 88.9, B = 80.5, y = 89.7 . A complete X-ray diffraction data set has been collected to 1.8 A ? resolution using cryocooling conditions and synchrotron radiation.

KW - bacterial chemotaxis

KW - methyl-accepting protein

KW - receptor

KW - sensing domain

UR - http://www.ncbi.nlm.nih.gov/pubmed/27139830

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