The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration

Yen Mei Chow, Beng Ti Tey, Mohd Nordin Ibrahim, Arbakariya Ariff, Tau Chuan Ling

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

Original languageEnglish
Pages (from-to)466-469
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Volume11
Issue number5
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

Keywords

  • Anion exchange
  • Biomass
  • Expanded bed adsorption
  • G6PDH
  • Yeast

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