The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration

Yen Mei Chow; Beng Ti Tey; Mohd Nordin Ibrahim; Arbakariya Ariff; Tau Chuan Ling

doi:10.1007/BF02932316

The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration

Yen Mei Chow, Beng Ti Tey, Mohd Nordin Ibrahim, Arbakariya Ariff, Tau Chuan Ling

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

Original language	English
Pages (from-to)	466-469
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	11
Issue number	5
DOIs	https://doi.org/10.1007/BF02932316
Publication status	Published - Sept 2006
Externally published	Yes

Keywords

Anion exchange
Biomass
Expanded bed adsorption
G6PDH
Yeast

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10.1007/BF02932316

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title = "The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration",

abstract = "The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).",

keywords = "Anion exchange, Biomass, Expanded bed adsorption, G6PDH, Yeast",

author = "Chow, {Yen Mei} and Tey, {Beng Ti} and Ibrahim, {Mohd Nordin} and Arbakariya Ariff and Ling, {Tau Chuan}",

year = "2006",

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doi = "10.1007/BF02932316",

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The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration. / Chow, Yen Mei; Tey, Beng Ti; Ibrahim, Mohd Nordin et al.
In: Biotechnology and Bioprocess Engineering, Vol. 11, No. 5, 09.2006, p. 466-469.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration

AU - Chow, Yen Mei

AU - Tey, Beng Ti

AU - Ibrahim, Mohd Nordin

AU - Ariff, Arbakariya

AU - Ling, Tau Chuan

PY - 2006/9

Y1 - 2006/9

N2 - The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

AB - The bed stability of Streamline DEAE (p = 1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

KW - Anion exchange

KW - Biomass

KW - Expanded bed adsorption

KW - G6PDH

KW - Yeast

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JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

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ER -

The performance of anion exchange expanded bed adsorption chromatography on the recovery of G6PDH from unclarified feedstock with high biomass concentration

Abstract

Keywords

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