The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport

Cenk Suphioglu; Damitha De Mel; Loveleen Kumar; Nadia Sadli; David Freestone; Agnes Michalczyk; Andrew Sinclair; M. Leigh Ackland

doi:10.1016/j.febslet.2009.12.013

The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport

Cenk Suphioglu, Damitha De Mel, Loveleen Kumar, Nadia Sadli, David Freestone, Agnes Michalczyk, Andrew Sinclair, M. Leigh Ackland

Research output: Contribution to journal › Article › Research › peer-review

26 Citations (Scopus)

Abstract

Docosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in neuronal cell membranes. We hypothesize that DHA induces a decrease in neuronal cell death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA-deficient medium increased the levels of active caspase-3, relative to levels in DHA-replete cells, confirming the adverse effects of DHA deficiency in promoting neuronal cell death. In DHA-treated M17 cells, zinc uptake was 65% less and ZnT3 mRNA and protein levels were reduced in comparison with DHA-depleted cells. We propose that the neuroprotective function of DHA is exerted through a reduction in cellular zinc levels that in turn inhibits apoptosis.

Original language	English
Pages (from-to)	612-618
Number of pages	7
Journal	FEBS Letters
Volume	584
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2009.12.013
Publication status	Published - 5 Feb 2010
Externally published	Yes

Keywords

Apoptosis
DHA
M17 human neuronal cell
Omega-3 fatty acid
Zinc
ZnT3

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10.1016/j.febslet.2009.12.013

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title = "The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport",

abstract = "Docosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in neuronal cell membranes. We hypothesize that DHA induces a decrease in neuronal cell death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA-deficient medium increased the levels of active caspase-3, relative to levels in DHA-replete cells, confirming the adverse effects of DHA deficiency in promoting neuronal cell death. In DHA-treated M17 cells, zinc uptake was 65% less and ZnT3 mRNA and protein levels were reduced in comparison with DHA-depleted cells. We propose that the neuroprotective function of DHA is exerted through a reduction in cellular zinc levels that in turn inhibits apoptosis.",

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T1 - The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport

AU - Suphioglu, Cenk

AU - De Mel, Damitha

AU - Kumar, Loveleen

AU - Sadli, Nadia

AU - Freestone, David

AU - Michalczyk, Agnes

AU - Sinclair, Andrew

AU - Ackland, M. Leigh

PY - 2010/2/5

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AB - Docosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in neuronal cell membranes. We hypothesize that DHA induces a decrease in neuronal cell death through reduced ZnT3 expression and zinc uptake. Exposure of M17 cells to DHA-deficient medium increased the levels of active caspase-3, relative to levels in DHA-replete cells, confirming the adverse effects of DHA deficiency in promoting neuronal cell death. In DHA-treated M17 cells, zinc uptake was 65% less and ZnT3 mRNA and protein levels were reduced in comparison with DHA-depleted cells. We propose that the neuroprotective function of DHA is exerted through a reduction in cellular zinc levels that in turn inhibits apoptosis.

KW - Apoptosis

KW - DHA

KW - M17 human neuronal cell

KW - Omega-3 fatty acid

KW - Zinc

KW - ZnT3

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The omega-3 fatty acid, DHA, decreases neuronal cell death in association with altered zinc transport

Abstract

Keywords

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