Abstract
Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.
| Original language | English |
|---|---|
| Pages (from-to) | 2452-2475 |
| Number of pages | 24 |
| Journal | Journal of Experimental Biology |
| Volume | 220 |
| Issue number | 13 |
| DOIs | |
| Publication status | Published - 1 Jul 2017 |
Keywords
- Feeding
- Learning and memory
- Locomotion
- Navigation
- Sensory processing
Cite this
}
The Ol1mpiad : Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae. / Almeida de Carvalho, Maria João; Berh, Dimitri; Braun, Andreas; Chen, Yi Chun; Eichler, Katharina; Eschbach, Claire; Fritsch, Pauline M.J.; Gerber, Bertram; Hoyer, Nina; Jiang, Xiaoyi; Kleber, Jörg; Klämbt, Christian; König, Christian; Louis, Matthieu; Michels, Birgit; Miroschnikow, Anton; Mirth, Christen; Miura, Daisuke; Niewalda, Thomas; Otto, Nils; Paisios, Emmanouil; Pankratz, Michael J.; Petersen, Meike; Ramsperger, Noel; Randel, Nadine; Risse, Benjamin; Saumweber, Timo; Schlegel, Philipp; Schleyer, Michael; Soba, Peter; Sprecher, Simon G.; Tanimura, Teiichi; Thum, Andreas S.; Toshima, Naoko; Truman, Jim W.; Yarali, Ayse; Zlatic, Marta.
In: Journal of Experimental Biology, Vol. 220, No. 13, 01.07.2017, p. 2452-2475.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - The Ol1mpiad
T2 - Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae
AU - Almeida de Carvalho, Maria João
AU - Berh, Dimitri
AU - Braun, Andreas
AU - Chen, Yi Chun
AU - Eichler, Katharina
AU - Eschbach, Claire
AU - Fritsch, Pauline M.J.
AU - Gerber, Bertram
AU - Hoyer, Nina
AU - Jiang, Xiaoyi
AU - Kleber, Jörg
AU - Klämbt, Christian
AU - König, Christian
AU - Louis, Matthieu
AU - Michels, Birgit
AU - Miroschnikow, Anton
AU - Mirth, Christen
AU - Miura, Daisuke
AU - Niewalda, Thomas
AU - Otto, Nils
AU - Paisios, Emmanouil
AU - Pankratz, Michael J.
AU - Petersen, Meike
AU - Ramsperger, Noel
AU - Randel, Nadine
AU - Risse, Benjamin
AU - Saumweber, Timo
AU - Schlegel, Philipp
AU - Schleyer, Michael
AU - Soba, Peter
AU - Sprecher, Simon G.
AU - Tanimura, Teiichi
AU - Thum, Andreas S.
AU - Toshima, Naoko
AU - Truman, Jim W.
AU - Yarali, Ayse
AU - Zlatic, Marta
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.
AB - Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.
KW - Feeding
KW - Learning and memory
KW - Locomotion
KW - Navigation
KW - Sensory processing
UR - http://www.scopus.com/inward/record.url?scp=85022021680&partnerID=8YFLogxK
U2 - 10.1242/jeb.156646
DO - 10.1242/jeb.156646
M3 - Article
AN - SCOPUS:85022021680
VL - 220
SP - 2452
EP - 2475
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
SN - 0022-0949
IS - 13
ER -