The Ol1mpiad: Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae

Maria João Almeida de Carvalho, Dimitri Berh, Andreas Braun, Yi Chun Chen, Katharina Eichler, Claire Eschbach, Pauline M.J. Fritsch, Bertram Gerber, Nina Hoyer, Xiaoyi Jiang, Jörg Kleber, Christian Klämbt, Christian König, Matthieu Louis, Birgit Michels, Anton Miroschnikow, Christen Mirth, Daisuke Miura, Thomas Niewalda, Nils OttoEmmanouil Paisios, Michael J. Pankratz, Meike Petersen, Noel Ramsperger, Nadine Randel, Benjamin Risse, Timo Saumweber, Philipp Schlegel, Michael Schleyer, Peter Soba, Simon G. Sprecher, Teiichi Tanimura, Andreas S. Thum, Naoko Toshima, Jim W. Truman, Ayse Yarali, Marta Zlatic

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.

Original languageEnglish
Pages (from-to)2452-2475
Number of pages24
JournalJournal of Experimental Biology
Volume220
Issue number13
DOIs
Publication statusPublished - 1 Jul 2017

Keywords

  • Feeding
  • Learning and memory
  • Locomotion
  • Navigation
  • Sensory processing

Cite this

Almeida de Carvalho, M. J., Berh, D., Braun, A., Chen, Y. C., Eichler, K., Eschbach, C., ... Zlatic, M. (2017). The Ol1mpiad: Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae. Journal of Experimental Biology, 220(13), 2452-2475. https://doi.org/10.1242/jeb.156646
Almeida de Carvalho, Maria João ; Berh, Dimitri ; Braun, Andreas ; Chen, Yi Chun ; Eichler, Katharina ; Eschbach, Claire ; Fritsch, Pauline M.J. ; Gerber, Bertram ; Hoyer, Nina ; Jiang, Xiaoyi ; Kleber, Jörg ; Klämbt, Christian ; König, Christian ; Louis, Matthieu ; Michels, Birgit ; Miroschnikow, Anton ; Mirth, Christen ; Miura, Daisuke ; Niewalda, Thomas ; Otto, Nils ; Paisios, Emmanouil ; Pankratz, Michael J. ; Petersen, Meike ; Ramsperger, Noel ; Randel, Nadine ; Risse, Benjamin ; Saumweber, Timo ; Schlegel, Philipp ; Schleyer, Michael ; Soba, Peter ; Sprecher, Simon G. ; Tanimura, Teiichi ; Thum, Andreas S. ; Toshima, Naoko ; Truman, Jim W. ; Yarali, Ayse ; Zlatic, Marta. / The Ol1mpiad : Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae. In: Journal of Experimental Biology. 2017 ; Vol. 220, No. 13. pp. 2452-2475.
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abstract = "Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.",
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Almeida de Carvalho, MJ, Berh, D, Braun, A, Chen, YC, Eichler, K, Eschbach, C, Fritsch, PMJ, Gerber, B, Hoyer, N, Jiang, X, Kleber, J, Klämbt, C, König, C, Louis, M, Michels, B, Miroschnikow, A, Mirth, C, Miura, D, Niewalda, T, Otto, N, Paisios, E, Pankratz, MJ, Petersen, M, Ramsperger, N, Randel, N, Risse, B, Saumweber, T, Schlegel, P, Schleyer, M, Soba, P, Sprecher, SG, Tanimura, T, Thum, AS, Toshima, N, Truman, JW, Yarali, A & Zlatic, M 2017, 'The Ol1mpiad: Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae', Journal of Experimental Biology, vol. 220, no. 13, pp. 2452-2475. https://doi.org/10.1242/jeb.156646

The Ol1mpiad : Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae. / Almeida de Carvalho, Maria João; Berh, Dimitri; Braun, Andreas; Chen, Yi Chun; Eichler, Katharina; Eschbach, Claire; Fritsch, Pauline M.J.; Gerber, Bertram; Hoyer, Nina; Jiang, Xiaoyi; Kleber, Jörg; Klämbt, Christian; König, Christian; Louis, Matthieu; Michels, Birgit; Miroschnikow, Anton; Mirth, Christen; Miura, Daisuke; Niewalda, Thomas; Otto, Nils; Paisios, Emmanouil; Pankratz, Michael J.; Petersen, Meike; Ramsperger, Noel; Randel, Nadine; Risse, Benjamin; Saumweber, Timo; Schlegel, Philipp; Schleyer, Michael; Soba, Peter; Sprecher, Simon G.; Tanimura, Teiichi; Thum, Andreas S.; Toshima, Naoko; Truman, Jim W.; Yarali, Ayse; Zlatic, Marta.

In: Journal of Experimental Biology, Vol. 220, No. 13, 01.07.2017, p. 2452-2475.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The Ol1mpiad

T2 - Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae

AU - Almeida de Carvalho, Maria João

AU - Berh, Dimitri

AU - Braun, Andreas

AU - Chen, Yi Chun

AU - Eichler, Katharina

AU - Eschbach, Claire

AU - Fritsch, Pauline M.J.

AU - Gerber, Bertram

AU - Hoyer, Nina

AU - Jiang, Xiaoyi

AU - Kleber, Jörg

AU - Klämbt, Christian

AU - König, Christian

AU - Louis, Matthieu

AU - Michels, Birgit

AU - Miroschnikow, Anton

AU - Mirth, Christen

AU - Miura, Daisuke

AU - Niewalda, Thomas

AU - Otto, Nils

AU - Paisios, Emmanouil

AU - Pankratz, Michael J.

AU - Petersen, Meike

AU - Ramsperger, Noel

AU - Randel, Nadine

AU - Risse, Benjamin

AU - Saumweber, Timo

AU - Schlegel, Philipp

AU - Schleyer, Michael

AU - Soba, Peter

AU - Sprecher, Simon G.

AU - Tanimura, Teiichi

AU - Thum, Andreas S.

AU - Toshima, Naoko

AU - Truman, Jim W.

AU - Yarali, Ayse

AU - Zlatic, Marta

PY - 2017/7/1

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N2 - Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.

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Almeida de Carvalho MJ, Berh D, Braun A, Chen YC, Eichler K, Eschbach C et al. The Ol1mpiad: Concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae. Journal of Experimental Biology. 2017 Jul 1;220(13):2452-2475. https://doi.org/10.1242/jeb.156646