The M3 muscarinic receptor mediates acetylcholine-induced cortisol secretion from bovine adrenocortical zona fasciculata/reticularis cells

Colin D. Clyne, Simon W. Walker, Moira R. Nicol, Brent C. Williams

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Abstract

In order to characterize the receptor subtype mediating acetylcholine (ACh)-induced cortisol secretion from purified bovine adrenocortical zona fasciculata/reticularis cells in primary culture, the potencies of a range of selective muscarinic antagonists of ACh-induced steroidogenesis were assessed by Schild analysis. Basal secretion of cortisol was 10.2 ± 1.4 pmol/well/30min. ACh stimulated a dose-dependent increase in cortisol secretion and was maximally effective at 10-5 M, at which concentration cortisol secretion was 143.4 ± 12.9 pmol/well/30 min. Hexahydro-sila-difenidol and para-fluoro-hexa-hydro-sila-difenidol were potent competitive antagonists of ACh-stimulated cortisol secretion, with pA2 values of 8.68 ± 0.28 and 7.96 ± 0.29, respectively. Pirenzepine (pA2 = 6.95 ± 0.28) and methoctramine (pA2 = 6.06 ± 0.27) were relatively weak competitive antagonists. The pA2 values determined in this study are characteristic of the M3 muscarinic receptor, and we conclude that this receptor subtype mediates ACh-induced cortisol secretion from bovine zona fasciculata/reticularis cells.

Original languageEnglish
Pages (from-to)1145-1150
Number of pages6
JournalBiochemical Pharmacology
Volume47
Issue number7
DOIs
Publication statusPublished - 29 Mar 1994
Externally publishedYes

Keywords

  • adrenal contex
  • bovine
  • cetylcholine
  • M receptor

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