The mouse relaxin gene: Nucleotide sequence and expression

B. A. Evans, M. John, K. J. Fowler, R. J. Summers, M. Cronk, J. Shine, G. W. Tregear

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

Relaxin is a polypeptide hormone that has a variety of physiological effects both on remodelling of collagen and on uterine contractility. These are most apparent during pregnancy. The sequences of relaxin cDNAs derived from ovaries of late-pregnant random-bred Swiss mice have been established. Multiple subclones obtained from three independent polymerase chain reaction experiments were found to encode relaxins which were identical except at position 11 in the A chain (Ile or Val). All mouse relaxin cDNAs expressed in the ovary during pregnancy had an extra tyrosine inserted prior to the final A chain cysteine residue, a result confirmed by direct sequencing of relaxin peptides. Whilst this tyrosine insertion must have local effects on the folding of the A chain, structure-activity studies will clarify whether it perturbs functional interaction with the relaxin receptor. We have shown that there is a single relaxin gene in the mouse genome, and that expression during pregnancy occurs in the ovary but is not detectable in the placenta, uterus or fetus.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalJournal of Molecular Endocrinology
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Jan 1993

Cite this