TY - JOUR
T1 - The Mouse Gastrointestinal Bacteria Catalogue enables translation between the mouse and human gut microbiotas via functional mapping
AU - Beresford-Jones, Benjamin S.
AU - Forster, Samuel C.
AU - Stares, Mark D.
AU - Notley, George
AU - Viciani, Elisa
AU - Browne, Hilary P.
AU - Boehmler, Daniel J.
AU - Soderholm, Amelia T.
AU - Kumar, Nitin
AU - Vervier, Kevin
AU - Cross, Justin R.
AU - Almeida, Alexandre
AU - Lawley, Trevor D.
AU - Pedicord, Virginia A.
N1 - Funding Information:
V.A.P. is supported by Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (206245/Z/17/Z). B.S.B.-J. is supported by a studentship from the Rosetrees Trust (A2194). T.D.L. is supported by the Wellcome Trust (098051). We thank Rahul Roychoudhuri and Paula Kuo from the Babraham Institute for providing mouse feces for metagenomic sequencing. B.S.B.-J. T.D.L. and V.A.P. conceived the study. S.C.F. initiated culturing of isolates. M.D.S. G.N. E.V. H.P.B. and B.S.B.-J. cultured isolates. B.S.B.-J. curated genomes and metagenomes, generated MAGs, performed computational analyses, and analyzed and assembled data. D.J.B. and J.R.C. performed and analyzed metabolomics. A.T.S. and B.S.B.-J. provided metagenomes from Wellcome Sanger Institute. N.K. K.V. and A.A. advised on computational analyses. A.A. and B.S.B.-J. wrote code for computational analyses. B.S.B.-J. T.D.L. and V.A.P. wrote the article. T.D.L. is a founder and CSO of Microbiotica. The other authors declare no competinginterests.
Funding Information:
V.A.P. is supported by Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (206245/Z/17/Z). B.S.B.-J. is supported by a studentship from the Rosetrees Trust (A2194). T.D.L. is supported by the Wellcome Trust (098051). We thank Rahul Roychoudhuri and Paula Kuo from the Babraham Institute for providing mouse feces for metagenomic sequencing.
Publisher Copyright:
© 2021 The Authors
PY - 2022/1/12
Y1 - 2022/1/12
N2 - Human health and disease have increasingly been shown to be impacted by the gut microbiota, and mouse models are essential for investigating these effects. However, the compositions of human and mouse gut microbiotas are distinct, limiting translation of microbiota research between these hosts. To address this, we constructed the Mouse Gastrointestinal Bacteria Catalogue (MGBC), a repository of 26,640 high-quality mouse microbiota-derived bacterial genomes. This catalog enables species-level analyses for mapping functions of interest and identifying functionally equivalent taxa between the microbiotas of humans and mice. We have complemented this with a publicly deposited collection of 223 bacterial isolates, including 62 previously uncultured species, to facilitate experimental investigation of individual commensal bacteria functions in vitro and in vivo. Together, these resources provide the ability to identify and test functionally equivalent members of the host-specific gut microbiotas of humans and mice and support the informed use of mouse models in human microbiota research.
AB - Human health and disease have increasingly been shown to be impacted by the gut microbiota, and mouse models are essential for investigating these effects. However, the compositions of human and mouse gut microbiotas are distinct, limiting translation of microbiota research between these hosts. To address this, we constructed the Mouse Gastrointestinal Bacteria Catalogue (MGBC), a repository of 26,640 high-quality mouse microbiota-derived bacterial genomes. This catalog enables species-level analyses for mapping functions of interest and identifying functionally equivalent taxa between the microbiotas of humans and mice. We have complemented this with a publicly deposited collection of 223 bacterial isolates, including 62 previously uncultured species, to facilitate experimental investigation of individual commensal bacteria functions in vitro and in vivo. Together, these resources provide the ability to identify and test functionally equivalent members of the host-specific gut microbiotas of humans and mice and support the informed use of mouse models in human microbiota research.
KW - bacteria culture collection
KW - butyrate
KW - commensal bacteria
KW - functionally equivalent species
KW - gut microbiota
KW - microbial drug metabolism
KW - mouse gut metagenomes
KW - mouse models
KW - public database
KW - translation between mouse and human
UR - http://www.scopus.com/inward/record.url?scp=85122629891&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2021.12.003
DO - 10.1016/j.chom.2021.12.003
M3 - Article
C2 - 34971560
AN - SCOPUS:85122629891
SN - 1931-3128
VL - 30
SP - 124-138.e8
JO - Cell Host & Microbe
JF - Cell Host & Microbe
IS - 1
ER -
