The mouse as a model for pulmonary Legionella infection

Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel, Elizabeth L. Hartland

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Infection of C57BL/6 mice with wild-type Legionella pneumophila typically results in very mild disease. However, in mice where the cytosolic recognition of flagellin is impaired by mutation, L. pneumophila infection results in more severe lung inflammation that is reminiscent of Legionnaires’ disease. This can be replicated in wild-type mice by using aflagellated mutants of L. pneumophila. These models greatly facilitate the investigation of L. pneumophila virulence factors and the complex pulmonary immune system that is triggered by infection. Here we describe methods for infecting C57BL/6 mice with aflagellated L. pneumophila, the quantification of bacterial load in the lungs and isolation and analysis of invading immune cells. These assays enable the identification of phagocyte subsets and can determine whether phagocytic cells act as a replicative niche for L. pneumophila replication.

Original languageEnglish
Title of host publicationLegionella
Subtitle of host publicationMethods and Protocols
EditorsCarmen Buchrieser, Hubert Hilbi
Place of PublicationNew York, NY
PublisherHumana Press
Chapter25
Pages399-417
Number of pages19
Edition2nd
ISBN (Electronic)9781493990481
ISBN (Print)9781493990474
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1921
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bacterial infection
  • Flow cytometry
  • Legionella
  • Mucosal immunology
  • Phagocytes
  • Pneumonia

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