The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK)

K. Winkel, T. Carroll, N. A. Williamson, V. Ignjatovic, K. Fung, A. W. Purcell, B. G. Fry

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

The Snake Venom Detection Kit (SVDK) is of major medical importance in Australia, yet it has never been rigorously characterised in terms of its sensitivity and specificity, especially when it comes to reports of false-negative and false-positive results. This study investigates reactions and cross-reactions of five venoms the SVDK is directed against and a number of purified toxins. Snakes showing the closest evolutionary relationships demonstrated the lowest level of cross-reactivity between groups. This was, instead, far more evident between snakes that are extraordinarily evolutionary separated. These snakes: Pseudechis australis, Acanthophis antarcticus and Notechis scutatus, in fact displayed more false-positive results. Examination of individual toxin groups showed that phospholipase A2s (PLA2s) tends to react strongly and display considerable cross-reactivity across groups while the three-finger toxins (3FTx) reacted poorly in all but the Acanthophis well. The hook effect was evident for all venoms, particularly Oxyuranus scutellatus. The results of this study show considerable variation in toxin detection, with implications in further development of venom detection, both in Australia and other countries.

Original languageEnglish
Pages (from-to)1041-1052
Number of pages12
JournalToxicon
Volume50
Issue number8
DOIs
Publication statusPublished - 15 Dec 2007
Externally publishedYes

Keywords

  • Antibody
  • Antivenom
  • ELISA
  • Protein
  • Venom

Cite this

Winkel, K., Carroll, T., Williamson, N. A., Ignjatovic, V., Fung, K., Purcell, A. W., & Fry, B. G. (2007). The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK). Toxicon, 50(8), 1041-1052. https://doi.org/10.1016/j.toxicon.2007.07.023
Winkel, K. ; Carroll, T. ; Williamson, N. A. ; Ignjatovic, V. ; Fung, K. ; Purcell, A. W. ; Fry, B. G. / The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK). In: Toxicon. 2007 ; Vol. 50, No. 8. pp. 1041-1052.
@article{1080c30833974412a708e83034956dcd,
title = "The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK)",
abstract = "The Snake Venom Detection Kit (SVDK) is of major medical importance in Australia, yet it has never been rigorously characterised in terms of its sensitivity and specificity, especially when it comes to reports of false-negative and false-positive results. This study investigates reactions and cross-reactions of five venoms the SVDK is directed against and a number of purified toxins. Snakes showing the closest evolutionary relationships demonstrated the lowest level of cross-reactivity between groups. This was, instead, far more evident between snakes that are extraordinarily evolutionary separated. These snakes: Pseudechis australis, Acanthophis antarcticus and Notechis scutatus, in fact displayed more false-positive results. Examination of individual toxin groups showed that phospholipase A2s (PLA2s) tends to react strongly and display considerable cross-reactivity across groups while the three-finger toxins (3FTx) reacted poorly in all but the Acanthophis well. The hook effect was evident for all venoms, particularly Oxyuranus scutellatus. The results of this study show considerable variation in toxin detection, with implications in further development of venom detection, both in Australia and other countries.",
keywords = "Antibody, Antivenom, ELISA, Protein, Venom",
author = "K. Winkel and T. Carroll and Williamson, {N. A.} and V. Ignjatovic and K. Fung and Purcell, {A. W.} and Fry, {B. G.}",
year = "2007",
month = "12",
day = "15",
doi = "10.1016/j.toxicon.2007.07.023",
language = "English",
volume = "50",
pages = "1041--1052",
journal = "Toxicon : official journal of the International Society on Toxinology",
issn = "0041-0101",
publisher = "Elsevier",
number = "8",

}

Winkel, K, Carroll, T, Williamson, NA, Ignjatovic, V, Fung, K, Purcell, AW & Fry, BG 2007, 'The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK)', Toxicon, vol. 50, no. 8, pp. 1041-1052. https://doi.org/10.1016/j.toxicon.2007.07.023

The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK). / Winkel, K.; Carroll, T.; Williamson, N. A.; Ignjatovic, V.; Fung, K.; Purcell, A. W.; Fry, B. G.

In: Toxicon, Vol. 50, No. 8, 15.12.2007, p. 1041-1052.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - The molecular basis of cross-reactivity in the Australian Snake Venom Detection Kit (SVDK)

AU - Winkel, K.

AU - Carroll, T.

AU - Williamson, N. A.

AU - Ignjatovic, V.

AU - Fung, K.

AU - Purcell, A. W.

AU - Fry, B. G.

PY - 2007/12/15

Y1 - 2007/12/15

N2 - The Snake Venom Detection Kit (SVDK) is of major medical importance in Australia, yet it has never been rigorously characterised in terms of its sensitivity and specificity, especially when it comes to reports of false-negative and false-positive results. This study investigates reactions and cross-reactions of five venoms the SVDK is directed against and a number of purified toxins. Snakes showing the closest evolutionary relationships demonstrated the lowest level of cross-reactivity between groups. This was, instead, far more evident between snakes that are extraordinarily evolutionary separated. These snakes: Pseudechis australis, Acanthophis antarcticus and Notechis scutatus, in fact displayed more false-positive results. Examination of individual toxin groups showed that phospholipase A2s (PLA2s) tends to react strongly and display considerable cross-reactivity across groups while the three-finger toxins (3FTx) reacted poorly in all but the Acanthophis well. The hook effect was evident for all venoms, particularly Oxyuranus scutellatus. The results of this study show considerable variation in toxin detection, with implications in further development of venom detection, both in Australia and other countries.

AB - The Snake Venom Detection Kit (SVDK) is of major medical importance in Australia, yet it has never been rigorously characterised in terms of its sensitivity and specificity, especially when it comes to reports of false-negative and false-positive results. This study investigates reactions and cross-reactions of five venoms the SVDK is directed against and a number of purified toxins. Snakes showing the closest evolutionary relationships demonstrated the lowest level of cross-reactivity between groups. This was, instead, far more evident between snakes that are extraordinarily evolutionary separated. These snakes: Pseudechis australis, Acanthophis antarcticus and Notechis scutatus, in fact displayed more false-positive results. Examination of individual toxin groups showed that phospholipase A2s (PLA2s) tends to react strongly and display considerable cross-reactivity across groups while the three-finger toxins (3FTx) reacted poorly in all but the Acanthophis well. The hook effect was evident for all venoms, particularly Oxyuranus scutellatus. The results of this study show considerable variation in toxin detection, with implications in further development of venom detection, both in Australia and other countries.

KW - Antibody

KW - Antivenom

KW - ELISA

KW - Protein

KW - Venom

UR - http://www.scopus.com/inward/record.url?scp=36049052297&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2007.07.023

DO - 10.1016/j.toxicon.2007.07.023

M3 - Article

VL - 50

SP - 1041

EP - 1052

JO - Toxicon : official journal of the International Society on Toxinology

JF - Toxicon : official journal of the International Society on Toxinology

SN - 0041-0101

IS - 8

ER -