The molecular assembly of the marsupial γμ T cell receptor defines a third T cell lineage

Kimberly A. Morrissey; Marcin Wegrecki; T. Praveena; Victoria L. Hansen; Lijing Bu; Komagal Kannan Sivaraman; Samuel Darko; Daniel C. Douek; Jamie Rossjohn; Robert D. Miller; Jérôme Le Nours

doi:10.1126/science.abe7070

The molecular assembly of the marsupial γμ T cell receptor defines a third T cell lineage

Kimberly A. Morrissey, Marcin Wegrecki, T. Praveena, Victoria L. Hansen, Lijing Bu, Komagal Kannan Sivaraman, Samuel Darko, Daniel C. Douek, Jamie Rossjohn, Robert D. Miller, Jérôme Le Nours

Research output: Contribution to journal › Article › Research › peer-review

21 Citations (Scopus)

Abstract

αβ and γδ T cell receptors (TCRs) are highly diverse antigen receptors that define two evolutionary conserved T cell lineages. We describe a population of γμTCRs found exclusively in non-eutherian mammals that consist of a two-domain (Vγ-Cγ) γ-chain paired to a three-domain (Vμ-Vμj-Cμ) μ-chain. γμTCRs were characterized by restricted diversity in the Vγ and Vμj domains and a highly diverse unpaired Vμ domain. Crystal structures of two distinct γμTCRs revealed the structural basis of the association of the γμTCR heterodimer. The γμ domain shared the characteristics of a single-domain antibody within which the hypervariable CDR3μ loop suggests a major antigen recognition determinant. We define here the molecular basis underpinning the assembly of a third TCR lineage, the γμTCR.

Original language	English
Pages (from-to)	1383-1388
Number of pages	6
Journal	Science
Volume	371
Issue number	6536
DOIs	https://doi.org/10.1126/science.abe7070
Publication status	Published - 26 Mar 2021

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10.1126/science.abe7070

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title = "The molecular assembly of the marsupial γμ T cell receptor defines a third T cell lineage",

abstract = "αβ and γδ T cell receptors (TCRs) are highly diverse antigen receptors that define two evolutionary conserved T cell lineages. We describe a population of γμTCRs found exclusively in non-eutherian mammals that consist of a two-domain (Vγ-Cγ) γ-chain paired to a three-domain (Vμ-Vμj-Cμ) μ-chain. γμTCRs were characterized by restricted diversity in the Vγ and Vμj domains and a highly diverse unpaired Vμ domain. Crystal structures of two distinct γμTCRs revealed the structural basis of the association of the γμTCR heterodimer. The γμ domain shared the characteristics of a single-domain antibody within which the hypervariable CDR3μ loop suggests a major antigen recognition determinant. We define here the molecular basis underpinning the assembly of a third TCR lineage, the γμTCR.",

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doi = "10.1126/science.abe7070",

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AU - Morrissey, Kimberly A.

AU - Wegrecki, Marcin

AU - Praveena, T.

AU - Hansen, Victoria L.

AU - Bu, Lijing

AU - Sivaraman, Komagal Kannan

AU - Darko, Samuel

AU - Douek, Daniel C.

AU - Rossjohn, Jamie

AU - Miller, Robert D.

AU - Le Nours, Jérôme

PY - 2021/3/26

Y1 - 2021/3/26

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AB - αβ and γδ T cell receptors (TCRs) are highly diverse antigen receptors that define two evolutionary conserved T cell lineages. We describe a population of γμTCRs found exclusively in non-eutherian mammals that consist of a two-domain (Vγ-Cγ) γ-chain paired to a three-domain (Vμ-Vμj-Cμ) μ-chain. γμTCRs were characterized by restricted diversity in the Vγ and Vμj domains and a highly diverse unpaired Vμ domain. Crystal structures of two distinct γμTCRs revealed the structural basis of the association of the γμTCR heterodimer. The γμ domain shared the characteristics of a single-domain antibody within which the hypervariable CDR3μ loop suggests a major antigen recognition determinant. We define here the molecular basis underpinning the assembly of a third TCR lineage, the γμTCR.

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The molecular assembly of the marsupial γμ T cell receptor defines a third T cell lineage

Abstract

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ARC Centre of Excellence in Advanced Molecular Imaging

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The molecular assembly of the marsupial γμ T cell receptor defines a third T cell lineage

Abstract

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ARC Centre of Excellence in Advanced Molecular Imaging

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