The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast

Trevor Lithgow, Tina Junne, Kitaru Suda, Sabine Gratzer, Gottfried Schatz

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Abstract

We have cloned the gene encoding the protein Mas22p, which spans the outer membrane of yeast mitochondria. Cells that completely lack Mas22p are inviable. The plasmid-borne MAS22 gene suppresses several defects resulting from the deletion of one or more of the mitochondrial protein import receptors. Defects of Mas20p-deficient cells are explained by the reduced level of Mas22p in these mutants. Mas22p has one acidic domain in the cytosol and a second acidic domain in the mitochondrial intermembrane space. We suggest that these domains of Mas22p on either side of the outer membrane function as a relay system for transferring the basic targeting sequences of precursor proteins into the mitochondria.

Original languageEnglish
Pages (from-to)11973-11977
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number25
DOIs
Publication statusPublished - 6 Dec 1994
Externally publishedYes

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