The migrating gubernaculum grows like a "limb bud"

Sophie Nightingale, Patrick Western, John Hutson

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)


BACKGROUND: The gubernaculum is crucial for testicular descent, and in the second, or inguinoscrotal, phase of descent it has no caudal attachments. Cranially, it is attached to the testis, but its caudal free tip migrates to the scrotum controlled by the genitofemoral nerve. Recent studies show active proliferation in the tip. We hypothesized that the gubernacular tip may grow like a limb bud. METHODS: We performed whole-mount in situ hybridization studies on male and female fetal mice (ages, E14.5-E18.5; n = 162) looking for limb bud regulatory factors. RESULTS: Our results showed that a member of the fibroblast growth factor (Fgf) family, Fgf10, and Hoxa10 were both expressed in the male gubernaculum at E14.5, and Hoxa10 was also expressed in the E16.5 mice. Weak staining was seen in the female gubernaculum for Hoxa10 on days E14.5 and E16.5, whereas no staining for Fgf10 was seen in the female gubernaculums. CONCLUSIONS: These studies, although preliminary, suggest limb bud regulators are essential for gubernacular growth. Hox genes and Fgfs may be fruitful areas of research to unravel the molecular control of gubernacular migration during testicular descent.
Original languageEnglish
Pages (from-to)387 - 390
Number of pages4
JournalJournal of Pediatric Surgery
Issue number2
Publication statusPublished - 2008
Externally publishedYes

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