TY - JOUR
T1 - The kiss/kissr systems are dispensable for zebrafish reproduction: Evidence from gene knockout studies
AU - Tang, Haipei
AU - Liu, Yun
AU - Luo, Daji
AU - Ogawa, Satoshi
AU - Yin, Yike
AU - Li, Shuisheng
AU - Zhang, Yong
AU - Hu, Wei
AU - Parhar, Ishwar
AU - Lin, Haoran
AU - Liu, Xiaochun
AU - Cheng, Christopher Hon Ki
PY - 2015
Y1 - 2015
N2 - The kiss1/gpr54 signaling system is considered to be a critical regulator of reproduction in most vertebrates. However, this presumption has not been tested vigorously in nonmammalian vertebrates. Distinct from mammals, multiple kiss1/gpr54 paralogous genes (kiss/kissr) have been identified in nonmammalian vertebrates, raising the possibility of functional redundancy among these genes. In this study, we have systematically generated the zebrafish kiss1-/-, kiss2-/-, and kiss1-/-; kiss2-/- mutant lines as well as the kissr1-/-, kissr2-/-, and kissr1-/-;kissr2-/- mutant lines using transcription activator-like effector nucleases. We have demonstrated that spermatogenesis and folliculogenesis as well as reproductive capability are not impaired in all of these 6 mutant lines. Collectively, our results indicate that kiss/kissr signaling is not absolutely required for zebrafish reproduction, suggesting that the kiss/kissr systems play nonessential roles for reproduction in certain nonmammalian vertebrates. These findings also demonstrated that fish and mammals have evolved different strategies for neuroendocrine control of reproduction.
AB - The kiss1/gpr54 signaling system is considered to be a critical regulator of reproduction in most vertebrates. However, this presumption has not been tested vigorously in nonmammalian vertebrates. Distinct from mammals, multiple kiss1/gpr54 paralogous genes (kiss/kissr) have been identified in nonmammalian vertebrates, raising the possibility of functional redundancy among these genes. In this study, we have systematically generated the zebrafish kiss1-/-, kiss2-/-, and kiss1-/-; kiss2-/- mutant lines as well as the kissr1-/-, kissr2-/-, and kissr1-/-;kissr2-/- mutant lines using transcription activator-like effector nucleases. We have demonstrated that spermatogenesis and folliculogenesis as well as reproductive capability are not impaired in all of these 6 mutant lines. Collectively, our results indicate that kiss/kissr signaling is not absolutely required for zebrafish reproduction, suggesting that the kiss/kissr systems play nonessential roles for reproduction in certain nonmammalian vertebrates. These findings also demonstrated that fish and mammals have evolved different strategies for neuroendocrine control of reproduction.
U2 - 10.1210/en.2014-1204
DO - 10.1210/en.2014-1204
M3 - Article
SN - 0013-7227
VL - 156
SP - 589
EP - 599
JO - Endocrinology
JF - Endocrinology
IS - 2
ER -