TY - JOUR
T1 - The isolation and characterization of γ3-melanotropin from the neurointermediary lobe of the rat pituitary
AU - Browne, C. A.
AU - Bennett, H. P.J.
AU - Solomon, S.
N1 - Funding Information:
The authors thank Susan Parkinson and Susan James for their fine technical assistance, and Dr. M. van der Rest and Mr. E. Wan of the Shriners Hospital, Montreal, for performing the amino acid analyses. This work was supported by MRC of Canada, grant MT-1658 and USPH grant HDO 4365.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1981/5/15
Y1 - 1981/5/15
N2 - γ3-Melanotropin has been isolated from the neurointermediary lobe of the rat pituitary by reversed-phase high performance liquid chromatography. Peptide mapping and amino acid analysis have demonstrated that γ3-melanotropin is a glycopeptide of 25 amino acids, which corresponds exactly to the γ3-melanotropin sequence predicted by DNA sequencing, with the addition of an extra lysine residue at the amino terminus. The carbohydrate chain is linked to the peptide between asparagine 16 and the carboxyl terminal glutamine 25, and contains 4 moles of glucosamine per mole of peptide but no galactosamine.
AB - γ3-Melanotropin has been isolated from the neurointermediary lobe of the rat pituitary by reversed-phase high performance liquid chromatography. Peptide mapping and amino acid analysis have demonstrated that γ3-melanotropin is a glycopeptide of 25 amino acids, which corresponds exactly to the γ3-melanotropin sequence predicted by DNA sequencing, with the addition of an extra lysine residue at the amino terminus. The carbohydrate chain is linked to the peptide between asparagine 16 and the carboxyl terminal glutamine 25, and contains 4 moles of glucosamine per mole of peptide but no galactosamine.
UR - http://www.scopus.com/inward/record.url?scp=0019805627&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(81)80101-5
DO - 10.1016/S0006-291X(81)80101-5
M3 - Article
C2 - 7259753
AN - SCOPUS:0019805627
VL - 100
SP - 336
EP - 343
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -