The isolation and characterization of γ3-melanotropin from the neurointermediary lobe of the rat pituitary

C. A. Browne, H. P.J. Bennett, S. Solomon

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γ3-Melanotropin has been isolated from the neurointermediary lobe of the rat pituitary by reversed-phase high performance liquid chromatography. Peptide mapping and amino acid analysis have demonstrated that γ3-melanotropin is a glycopeptide of 25 amino acids, which corresponds exactly to the γ3-melanotropin sequence predicted by DNA sequencing, with the addition of an extra lysine residue at the amino terminus. The carbohydrate chain is linked to the peptide between asparagine 16 and the carboxyl terminal glutamine 25, and contains 4 moles of glucosamine per mole of peptide but no galactosamine.

Original languageEnglish
Pages (from-to)336-343
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 15 May 1981
Externally publishedYes

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