The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid

Peter R. Dodd, Wendy E.J. Watson, Murray M. Morrison, Graham A.R. Johnston, A. Ian Smith, Anita Ruwoldt, Ronald S. Walls

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [3H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid.

Original languageEnglish
Pages (from-to)213-224
Number of pages12
JournalMetabolic Brain Disease
Volume6
Issue number4
DOIs
Publication statusPublished - 1 Dec 1991
Externally publishedYes

Keywords

  • glutamic acid
  • HPLC
  • Huntington disease
  • kainic acid
  • leukocytes
  • receptors

Cite this

Dodd, P. R., Watson, W. E. J., Morrison, M. M., Johnston, G. A. R., Smith, A. I., Ruwoldt, A., & Walls, R. S. (1991). The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid. Metabolic Brain Disease, 6(4), 213-224. https://doi.org/10.1007/BF00996921
Dodd, Peter R. ; Watson, Wendy E.J. ; Morrison, Murray M. ; Johnston, Graham A.R. ; Smith, A. Ian ; Ruwoldt, Anita ; Walls, Ronald S. / The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid. In: Metabolic Brain Disease. 1991 ; Vol. 6, No. 4. pp. 213-224.
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Dodd, PR, Watson, WEJ, Morrison, MM, Johnston, GAR, Smith, AI, Ruwoldt, A & Walls, RS 1991, 'The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid', Metabolic Brain Disease, vol. 6, no. 4, pp. 213-224. https://doi.org/10.1007/BF00996921

The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid. / Dodd, Peter R.; Watson, Wendy E.J.; Morrison, Murray M.; Johnston, Graham A.R.; Smith, A. Ian; Ruwoldt, Anita; Walls, Ronald S.

In: Metabolic Brain Disease, Vol. 6, No. 4, 01.12.1991, p. 213-224.

Research output: Contribution to journalArticleResearchpeer-review

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