The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid

Peter R. Dodd; Wendy E.J. Watson; Murray M. Morrison; Graham A.R. Johnston; A. Ian Smith; Anita Ruwoldt; Ronald S. Walls

doi:10.1007/BF00996921

The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid

Peter R. Dodd, Wendy E.J. Watson, Murray M. Morrison, Graham A.R. Johnston, A. Ian Smith, Anita Ruwoldt, Ronald S. Walls

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [³H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [³H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid.

Original language	English
Pages (from-to)	213-224
Number of pages	12
Journal	Metabolic Brain Disease
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF00996921
Publication status	Published - 1 Dec 1991
Externally published	Yes

Keywords

glutamic acid
HPLC
Huntington disease
kainic acid
leukocytes
receptors

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF00996921

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title = "The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid",

abstract = "A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [3H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid.",

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AU - Morrison, Murray M.

AU - Johnston, Graham A.R.

AU - Smith, A. Ian

AU - Ruwoldt, Anita

AU - Walls, Ronald S.

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AB - A factor from mammalian and human brain, which inhibits the rate of migration of leukocytes obtained from sufferers from Huntington disease (Walls and Ruwoldt, 1984), inhibited the specific binding of the neurotoxin [3H]kainic acid to rat brain synaptic membranes. The factor was present in sucrose-particulate but not in soluble fractions from rat sub-cortical tissue, and was destroyed by tryptic digestion. Whereas an ammonium sulfate fraction of direct saline extracts of brain (Walls and Ruwoldt, 1984) gave poor chromatography on HPLC, prior separation of a sucrose-particulate fraction resulted in much improved chromatography. There was a good concordance between leukocyte migration inhibitory activity and [3H]kainic acid binding inhibitory activity. The factor may be an endogenous modulator of the kainic acid subset of receptors for the excitatory neurotransmitter glutamic acid.

KW - glutamic acid

KW - HPLC

KW - Huntington disease

KW - kainic acid

KW - leukocytes

KW - receptors

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ER -

The interaction of a huntington disease factor with receptors for the neurotoxin kainic acid

Abstract

Keywords

UN SDGs

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