The influence of bakers' yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography

Yen Mei Chow; Beng Ti Tey; Mohd Nordin Ibrahim; Arbakariya Arif; Tau Chuan Ling

doi:10.1007/BF02932293

The influence of bakers' yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography

Yen Mei Chow, Beng Ti Tey, Mohd Nordin Ibrahim, Arbakariya Arif, Tau Chuan Ling

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

The influence of whole yeast cells (0-15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ = 1.5 g/cm³) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a C/C₀ = 0.1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent; respectively.

Original language	English
Pages (from-to)	552-555
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/BF02932293
Publication status	Published - Nov 2005
Externally published	Yes

Keywords

Bovine serum albumin
Cibacron Blue 3GA
Dye-ligand
Expanded bed
Yeast cells

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10.1007/BF02932293

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title = "The influence of bakers' yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography",

abstract = "The influence of whole yeast cells (0-15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ = 1.5 g/cm3) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a C/C0 = 0.1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent; respectively.",

keywords = "Bovine serum albumin, Cibacron Blue 3GA, Dye-ligand, Expanded bed, Yeast cells",

author = "Chow, {Yen Mei} and Tey, {Beng Ti} and Ibrahim, {Mohd Nordin} and Arbakariya Arif and Ling, {Tau Chuan}",

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year = "2005",

month = nov,

doi = "10.1007/BF02932293",

language = "English",

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AU - Ibrahim, Mohd Nordin

AU - Arif, Arbakariya

AU - Ling, Tau Chuan

N1 - Funding Information: ^ÅâåçïäÉÇÖÉãÉåí This work was funded by the IRPA grant (09-02-04-0621) and a new lecturer research scheme from the Ministry of Science, Technology and the Innovation, Malaysia. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2005/11

Y1 - 2005/11

N2 - The influence of whole yeast cells (0-15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ = 1.5 g/cm3) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a C/C0 = 0.1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent; respectively.

AB - The influence of whole yeast cells (0-15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ = 1.5 g/cm3) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a C/C0 = 0.1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent; respectively.

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KW - Cibacron Blue 3GA

KW - Dye-ligand

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JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 6

ER -

The influence of bakers' yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography

Abstract

Keywords

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