The influence of bakers' yeast cells on protein adsorption performance in dye-ligand expanded bed chromatography

Yen Mei Chow, Beng Ti Tey, Mohd Nordin Ibrahim, Arbakariya Arif, Tau Chuan Ling

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3 Citations (Scopus)


The influence of whole yeast cells (0-15% w/v) on the protein adsorption performance in dye-ligand chromatography was explored. The adsorption of a model protein, bovine serum albumin (BSA), was selected to demonstrate this approach. The UpFront adsorbent (ρ = 1.5 g/cm3) derivatised with Cibacron Blue 3GA and a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography, Denmark, were employed in the batch binding and expanded bed operation. The BSA binding capacity was demonstrated to not be adversely affected by the presence of yeast cells. The dynamic binding capacity of BSA at a C/C0 = 0.1 biomass concentration of 5, 10, 15% w/v were 9, 8, and 7.5 mg/mL of settled adsorbent; respectively.

Original languageEnglish
Pages (from-to)552-555
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Issue number6
Publication statusPublished - Nov 2005
Externally publishedYes


  • Bovine serum albumin
  • Cibacron Blue 3GA
  • Dye-ligand
  • Expanded bed
  • Yeast cells

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