The influence of baker's yeast cells on protein adsorption in anion exchange expanded bed chromatography

Chow Yen Mei, Tey Beng Ti, Mohammad Nordin Ibrahim, Arbakariya Ariff, Ling Tau Chuan

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)


The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE (ρ = 1.2 g/cm3) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

Original languageEnglish
Pages (from-to)280-283
Number of pages4
JournalBiotechnology and Bioprocess Engineering
Issue number3
Publication statusPublished - 2005
Externally publishedYes


  • Adsorbent
  • Binding sites
  • Bovine serum albumin
  • Expanded bed chromatography
  • Yeast cells

Cite this