The influence of baker's yeast cells on protein adsorption in anion exchange expanded bed chromatography

Chow Yen Mei; Tey Beng Ti; Mohammad Nordin Ibrahim; Arbakariya Ariff; Ling Tau Chuan

doi:10.1007/BF02932026

The influence of baker's yeast cells on protein adsorption in anion exchange expanded bed chromatography

Chow Yen Mei, Tey Beng Ti, Mohammad Nordin Ibrahim, Arbakariya Ariff, Ling Tau Chuan

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE (ρ = 1.2 g/cm³) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

Original language	English
Pages (from-to)	280-283
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	10
Issue number	3
DOIs	https://doi.org/10.1007/BF02932026
Publication status	Published - 2005
Externally published	Yes

Keywords

Adsorbent
Binding sites
Bovine serum albumin
Expanded bed chromatography
Yeast cells

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10.1007/BF02932026

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title = "The influence of baker's yeast cells on protein adsorption in anion exchange expanded bed chromatography",

abstract = "The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE (ρ = 1.2 g/cm3) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.",

keywords = "Adsorbent, Binding sites, Bovine serum albumin, Expanded bed chromatography, Yeast cells",

author = "Mei, {Chow Yen} and Ti, {Tey Beng} and Ibrahim, {Mohammad Nordin} and Arbakariya Ariff and Chuan, {Ling Tau}",

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year = "2005",

doi = "10.1007/BF02932026",

language = "English",

volume = "10",

pages = "280--283",

journal = "Biotechnology and Bioprocess Engineering",

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AU - Ti, Tey Beng

AU - Ibrahim, Mohammad Nordin

AU - Ariff, Arbakariya

AU - Chuan, Ling Tau

N1 - Funding Information: ^ÅâåçïäÉÇÖÉãÉåí This study was supported by the IRPA Grant (09-02-04-0621) from the Ministry of Science, Technology and the Environment, Malaysia. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2005

Y1 - 2005

N2 - The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE (ρ = 1.2 g/cm3) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

AB - The adsorption of a model protein bovine serum albumin (BSA) in expanded bed chromatography was undertaken by exploiting a commercially available expanded bed column (20 mm i.d.) from UpFront Chromatography and Streamline DEAE (ρ = 1.2 g/cm3) from Amersham Pharmacia Biotechnology. The influence of whole yeast cells on the adsorption capacity of column was explored by employing yeast cells in a concentration ranged of 0 to 15% (w/v). Equilibrium isotherms for adsorption of BSA on Streamline DEAE were correlated by using Langmuir equation. The presence of yeast cells resulted in decreased of BSA binding capacity in both batch binding and expanded bed chromatography. Results indicated that the yeast cells act as competitor for proteins to bind to the sites on adsorbents.

KW - Adsorbent

KW - Binding sites

KW - Bovine serum albumin

KW - Expanded bed chromatography

KW - Yeast cells

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DO - 10.1007/BF02932026

M3 - Article

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VL - 10

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EP - 283

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

SN - 1226-8372

IS - 3

ER -

The influence of baker's yeast cells on protein adsorption in anion exchange expanded bed chromatography

Abstract

Keywords

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