The implications of stochastic synthesis for the conjugation of functional groups to nanoparticles

Douglas G. Mullen, Ankur M. Desai, Jack N. Waddell, Xue Min Cheng, Christopher V. Kelly, Daniel Q. McNerny, István J. Majoras, James R. Baker, Leonard M. Sander, Bradford G. Orr, Mark M. Banaszak Holl

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Stochastic synthesis of a ligand coupled to a nanoparticle results in a distribution of populations with different numbers of ligands per nanoparticle. This distribution was resolved and quantified using HPLC and is in excellent agreement with the ligand/nanoparticle average measured by 1H NMR, gel permeation chromatography (GPC), and Potentiometrie titration, and yet significantly more disperse than commonly held perceptions of monodispersity. Two statistical models were employed to confirm that the observed heterogeneity is consistent with theoretical expectations.

Original languageEnglish
Pages (from-to)1748-1752
Number of pages5
JournalBioconjugate Chemistry
Issue number9
Publication statusPublished - 1 Sep 2008
Externally publishedYes

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