In this comparative study, the impact of two stand-alone university courses on pre-service teachers attitudes, concerns and teaching efficacy to teach in inclusive classrooms is examined. Twenty-eight preservice teachers (PSTs) from Australia and 60 PSTs from Canada completed a survey at pre- and poststages of the course. It was found that after completion of the course, Australian participants attitudes improved, their concerns declined, and they became more confident in their ability to teach in inclusive classrooms. Similarly, Canadian participants concerns declined and their teaching efficacy improved, but they became more apprehensive about teaching in inclusive classrooms. Relationships among the three variables (attitudes, concerns and teaching efficacy) were examined at pre- and post-test stages for both cohorts to understand how they interacted within each of the two countries. Implications of the findings for teacher education are discussed.