The identification and cloning of the murine genes encoding the liver specific F alloantigens

Suzanne S. Teuber, Ross L. Coppel, Aftab A. Ansari, Patrick S.C. Leung, Rachel Neve, Ian R. Mackay, M. Eric Gershwin

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Abstract

The liver specific F alloantigen is a highly conserved abundant protein found in hepatic cytoplasm; smaller amounts are detected in renal tubule cells and the perikaryon cells of the central nervous system. Although the biological function of the F alloantigen is unknown, the immune response to F has been extensively studied as a murine model of tolerance and autoimmunity. Murine F exists in two allelic forms, designated F type 1 and type 2, each of approximately 43 kDa. The immune response to the allotypic form is restricted to mouse strains of I-Ak. Responding strains immunized with allotypic F break tolerance and produce precipitating antibody that reacts with both allelic forms, i.e., immunogen and self. Thus an autoantibody is produced. Using the previously isolated rat F cDNA as a probe, we report the cloning and sequencing of the two murine F allotypes. These two alleles are nearly homologous except at the extremes of the coding sequence. There are a number of regions within the F sequence that are similar to peptides that interact specifically with I-Ak. In particular, there is a sequence near the carboxy terminus, where the two allotypes differ, that has homology to the I-Ak restricted malarial antigen peptide of the ring-infected erythrocyte surface antigen (RESA).

Original languageEnglish
Pages (from-to)857-870
Number of pages14
JournalJournal of Autoimmunity
Volume4
Issue number6
DOIs
Publication statusPublished - 1 Jan 1991

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