The identification and characterization of α-N-acetylated β-endorphin in the human pituitary gland

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Abstract

By using a radioimmunoassay specific for α-N-acetyl β-endorphin and its C-terminally shortened forms, we have established the presence of immunoreactive α-N-acetyl endorphin (irNacEP) in extracts of five postmortem human pituitary glands (2.27 ± 0.64 ng/gland). This immunoreactivity has been further characterized by subjecting these extracts to reverse-phase high-performance liquid chromatography (RP-HPLC). In all cases the major peaks of irNacEP co-migrated with synthetic human standard α-N-acetyl α-endorphin (NacαEP), α-N-acetyl γ-endorphin (NacγEP) and NacβEP. These studies thus represent the initial demonstration that α-N-acetylation of β-endorphin and its shorter molecular forms occurs in the human pituitary gland.

Original languageEnglish
Pages (from-to)109-111
Number of pages3
JournalFEBS Letters
Volume185
Issue number1
DOIs
Publication statusPublished - 3 Jun 1985
Externally publishedYes

Keywords

  • Acetylation
  • Human pituitary
  • Radioimmunoassay
  • Reverse-phase HPLC
  • β-Endorphin

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